A transcriptomic study of selenium against liver injury induced by beta-cypermethrin in mice by RNA-seq
| Autor: | Bei Huang, Huiqiu Zhu, Xianping Chen, Silin Yang, Qingyang Tang, Dahai Liu, Mengting Gong, Kan He |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecticides RNA-Seq Biology 01 natural sciences Transcriptome 03 medical and health sciences Mice Selenium Cytochrome P-450 Enzyme System Gene expression Pyrethrins Genetics medicine Animals Gene Janus Kinases Liver injury RNA General Medicine medicine.disease Molecular biology STAT Transcription Factors 030104 developmental biology Liver Liver function Signal transduction Chemical and Drug Induced Liver Injury 010606 plant biology & botany |
| Zdroj: | Functionalintegrative genomics. 20(3) |
| ISSN: | 1438-7948 |
| Popis: | Evidence from biochemical liver function index and histopathology analysis suggested that selenium could effectively repair the liver injury caused by beta-cypermethrin (β-CYP). However, the molecular mechanism of selenium against liver injury induced by β-CYP remains unclear. In the present study, dynamic changes in gene expression profiles before and after the treatment of Na2SeO3 in liver injury mice were analyzed by using RNA sequencing. As a result, several essential genes and pathways were identified to be significantly associated with this process. In particular, ten genes including Cyp2j11, Cyp2b10, Cyp3a13, Dhrs9, Socs2, Stat4, Gm13305, Cyp3a44, Retsat, and Cyp26b1 were significantly enriched in the functional categories related to retinol metabolism, linoleic acid metabolism, and Jak-STAT signaling pathway. Among them, the expression patterns of nine genes were validated by qRT-PCR, except for Cyp3a44. Furthermore, we have constructed the associated regulatory network based on the identified targets revealed by high throughput screening. Our study may provide insight into the molecular mechanism underlying the protective effect of selenium against liver injury induced by β-CYP in mammals. |
| Databáze: | OpenAIRE |
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