The N- and C-terminal boundaries of myelin basic protein determinants required for encephalitogenic and proliferative responses of Lewis rat T cells
| Autor: | Mark D. Mannie, D.C. U'Prichard, Philip Y. Paterson, G Flouret |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Adoptive cell transfer T cell Encephalomyelitis T-Lymphocytes Immunology Biology Cell Line Myelin Epitopes Antigen medicine Immunology and Allergy Animals Immunochemistry Immunization Passive Myelin Basic Protein T lymphocyte medicine.disease Molecular biology In vitro Myelin basic protein Rats medicine.anatomical_structure Neurology Rats Inbred Lew biology.protein Encephalitis Neurology (clinical) Peptides Cell Division |
| Zdroj: | Journal of neuroimmunology. 26(3) |
| ISSN: | 0165-5728 |
| Popis: | Highly purified synthetic peptides representing portions of the 68-86 sequence of guinea pig (GP) myelin basic protein (GPMBP) were used to define the N- and C-termini of encephalitogenic determinants that cause experimental autoimmune encephalomyelitis (EAE) in Lewis rats. Each peptide was tested for: (a) induction of EAE, (b)in vitro potentiation of EAE transfer activity by GPMBP-sensitized lymph node cells (LNC), (c) in vitro proliferation of GPMBP-sensitized LNC, and (d) in vitro proliferation of a GPMBP-reactive line of EAE-inducing T cells. In these bioassays, the general rank order of potency was: GPMBP greater than or equal to GP68-86 greater than or equal to GP72-86 greater than [G84]GP68-86 greater than or equal to GP68-84 much greater than GP75-85 greater than or equal to GP75-84 = virtually no activity. These results demonstrate that the encephalitogenic region is bounded by the 72-74 and 84-86 sequences. Further evidence presented herein indicates that the 75-84 sequence contains the primary antigenic features required for specific T cell recognition of the encephalitogenic region. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|