Metagenomic analysis of the gut microbiome in atherosclerosis patients identify cross-cohort microbial signatures and potential therapeutic target
| Autor: | Xueyan Liu, Wenjing Zhao, Sheng Liu, Lixin Cheng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male China Bacteroides xylanisolvens Gut flora Biochemistry Microbiology Cohort Studies 03 medical and health sciences chemistry.chemical_compound Feces 0302 clinical medicine Biosynthesis Roseburia inulinivorans Genetics Humans Glycolysis Roseburia intestinalis Molecular Biology Aged Sweden biology Bacteria Middle Aged biology.organism_classification Atherosclerosis Gastrointestinal Microbiome 030104 developmental biology chemistry Metagenomics Case-Control Studies Cohort Metabolome Metagenome Female 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 34(11) |
| ISSN: | 1530-6860 |
| Popis: | The gut microbiota is associated with cardiovascular diseases, including atherosclerosis. However, the composition, functional capacity, and metabolites of the gut microbiome about atherosclerosis have not been comprehensively studied. Here, we reanalyzed 25 metagenomic stool samples from Sweden and 385 metagenomic stool samples from China using HUMAnN2, PanPhlAn, and MelonnPan to obtain more sufficient information. We found that the samples from atherosclerotic patients in both cohorts were depleted in Bacteroides xylanisolvens, Odoribacter splanchnicus, Eubacterium eligens, Roseburia inulinivorans, and Roseburia intestinalis. At the functional level, healthy metagenomes were both enriched in pathways of starch degradation V, glycolysis III (from glucose), CDP-diacylglycerol biosynthesis, and folate transformations. R inulinivorans and R intestinalis are major contributors to starch degradation V, while E eligens greatly contribute to the pathway CDP-diacylglycerol biosynthesis, and B xylanisolvens and B uniformis contribute to folate transformations II. The 11 marker species selected from the Chinese cohort distinguish patients from controls with an area under the receiver operating characteristics curve (AUC) of 0.86. Strain-level microbial analysis revealed a geographically associated adaptation of the strains from E eligens, B uniformis, and E coli. Two gut microbial metabolites, nicotinic acid and hydrocinnamic acid, had significantly higher predicted abundance in the control samples compared to the patients in the Chinese cohort, and interestinglynicotinic acid is already an effective lipid-lowering drug to reducing cardiovascular risk. Our results indicate intestinal bacteria such as B xylanisolvens, E eligens, and R inulinivorans could be promising probiotics and potential therapeutic target for atherosclerosis. |
| Databáze: | OpenAIRE |
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