Genetic dissection of the zebrafish retinal stem-cell compartment
| Autor: | Herwig Baier, Jason R. Meyers, Wendy Staub, Pamela A. Raymond, Ann M. Wehman |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Cell type
Genetic Linkage Population Biology Cell cycle Retina Ciliary marginal zone medicine Animals Genetic Testing Progenitor cell education Cell Shape Molecular Biology Zebrafish Genetics education.field_of_study Stem cell Danio rerio Stem Cells Cell Biology biology.organism_classification Phenotype Embryonic stem cell Neural retina Cell biology medicine.anatomical_structure Mutation Retinal pigmented epithelium Developmental Biology |
| Zdroj: | Developmental Biology. (1):53-65 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2005.02.010 |
| Popis: | In a large-scale forward-genetic screen, we discovered that a limited number of genes are required for the regulation of retinal stem cells after embryogenesis in zebrafish. In 18 mutants out of almost 2000 F2 families screened, the eye undergoes normal embryonic development, but fails to continue growth from the ciliary marginal zone (CMZ), the post-embryonic stem-cell niche. Class I-A mutants (5 loci) display lower amounts of proliferation in the CMZ, while nearly all cells in the retina appear differentiated. Class I-B mutants (2 loci) have a reduced CMZ with a concomitant expansion in the retinal pigmented epithelium (RPE), suggesting a common post-embryonic stem cell is the source for these neighboring cell types. Class II encompasses three distinct types of mutants (11 loci) with expanded CMZ, in which the progenitor population is arrested in the cell cycle. We also show that in at least one combination, the reduced CMZ phenotype is genetically epistatic to the expanded CMZ phenotype, suggesting that Class I genes are more likely to affect the stem cells and Class II the progenitor cells. Finally, a comparative mapping analysis demonstrates that the new genes isolated do not correspond to genes previously implicated in stem-cell regulation. Our study suggests that embryonic and post-embryonic stem cells utilize separable genetic programs in the zebrafish retina. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|