Advances in nucleic acid-based detection methods
| Autor: | M J Wolcott |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Microbiology (medical)
Background information General Immunology and Microbiology Epidemiology Hybridization probe Public Health Environmental and Occupational Health Nucleic acid methods Nucleic Acid Hybridization Routine laboratory Nucleic acid amplification technique Biology Polymerase Chain Reaction Nucleic acid thermodynamics Infectious Diseases Biochemistry Nucleic Acids Laboratory Scientists Nucleic acid Humans DNA Probes Nucleic Acid Amplification Techniques Research Article |
| Zdroj: | Clinical Microbiology Reviews. 5:370-386 |
| ISSN: | 1098-6618 0893-8512 |
| DOI: | 10.1128/cmr.5.4.370 |
| Popis: | Laboratory techniques based on nucleic acid methods have increased in popularity over the last decade with clinical microbiologists and other laboratory scientists who are concerned with the diagnosis of infectious agents. This increase in popularity is a result primarily of advances made in nucleic acid amplification and detection techniques. Polymerase chain reaction, the original nucleic acid amplification technique, changed the way many people viewed and used nucleic acid techniques in clinical settings. After the potential of polymerase chain reaction became apparent, other methods of nucleic acid amplification and detection were developed. These alternative nucleic acid amplification methods may become serious contenders for application to routine laboratory analyses. This review presents some background information on nucleic acid analyses that might be used in clinical and anatomical laboratories and describes some recent advances in the amplification and detection of nucleic acids. |
| Databáze: | OpenAIRE |
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