Increased apelin receptor gene expression in the subfornical organ of spontaneously hypertensive rats
| Autor: | Julian F. R. Paton, Stephen J Lolait, Anne-Marie O'Carroll, Aoife O'Carroll-Lolait, Philip R. Griffiths, Aarifah Bijabhai |
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| Rok vydání: | 2020 |
| Předmět: |
Central Nervous System
Male 0301 basic medicine Gene Expression Blood Pressure Vascular Medicine Nervous System Rats Inbred WKY 0302 clinical medicine Animal Cells Rats Inbred SHR Medicine and Health Sciences In Situ Hybridization In Situ Hybridization Fluorescence Neurons Mammals Apelin Receptors Multidisciplinary Lamina terminalis Area postrema Eukaryota Animal Models Up-Regulation Apelin medicine.anatomical_structure Experimental Organism Systems Blood-Brain Barrier Hypertension Vertebrates Medicine Cellular Types Cellular Structures and Organelles Anatomy Research Article Subfornical Organ medicine.medical_specialty Imaging Techniques Science Molecular Probe Techniques Neuropeptide In situ hybridization Biology Research and Analysis Methods Blood–brain barrier Rodents 03 medical and health sciences Model Organisms Internal medicine Fluorescence Imaging Genetics medicine Animals Molecular Biology Techniques Molecular Biology Apelin receptor Organisms Biology and Life Sciences Cell Biology Probe Hybridization Subfornical organ Rats 030104 developmental biology Endocrinology Cell Signaling Structures Cellular Neuroscience Amniotes Animal Studies 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Griffiths, P R, Lolait, S J, Bijabhai, A, O'Carroll-Lolait, A, Paton, J F R & O'Carroll, A-M 2020, ' Increased apelin receptor gene expression in the subfornical organ of spontaneously hypertensive rats. ', PLoS ONE . https://doi.org/10.1371/journal.pone.0231844 PLoS ONE, Vol 15, Iss 4, p e0231844 (2020) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0231844 |
| Popis: | The vascular organ of the lamina terminalis, subfornical organ (SFO), and area postrema comprise the sensory circumventricular organs (CVO) which are central structures that lie outside the blood brain barrier and are thought to provide an interface between peripherally circulating signals and the brain through their projections to central autonomic structures. The SFO expresses mRNA for the G protein-coupled apelin receptor (APJ, gene name aplnr) and exogenous microinjection of the neuropeptide apelin (apln) to the SFO elicits a depressor effect. Here we investigated the expression and cellular distribution of aplnr, apln and the recently described ligand apela (apela) in the CVOs and investigated whether differences in the levels of expression of apelinergic gene transcripts in these regions might underlie the chronic elevated blood pressure seen in hypertension. We carried out multiplex in situ hybridization histochemistry on CVO tissue sections from spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) controls. Confocal immunofluorescent images indicated strong aplnr expression, with lower levels of apln and modest apela expression, in the CVOs of both WKY rats and SHRs, in both neurons and glia. The expression level of aplnr transcripts was increased in the SFO of SHRs compared to WKY rats. Our data may highlight a potential dysfunction in the communication between CVOs and downstream signalling pathways in SHRs, which may contribute to its different phenotype/s. |
| Databáze: | OpenAIRE |
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