The occurrence and genetic characterization of Cryptosporidium and Giardia species in foals in Belgium, The Netherlands, Germany and Greece
| Autor: | Nikolaos Voutzourakis, M. Looijen, Despoina Kostopoulou, Edwin Claerebout, Amimul Ehsan, Stijn Casaert, T. Doornaert, Janina Demeler, N. De Wilde, Anastasios Saratsis, D.C.K. van Doorn, Thomas Geurden, Smaragda Sotiraki, G. von Samson-Himmelstjerna, Nikolaos Tzanidakis |
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| Předmět: |
Diarrhea
Giardiasis Veterinary medicine Genotype animal diseases Cryptosporidiosis Cryptosporidium Microbiology parasitic diseases medicine Parasite hosting Animals Horses Direct fluorescent antibody Feces General Veterinary biology Giardia Zoonosis Horse General Medicine medicine.disease biology.organism_classification Animals Newborn Parasitology Horse Diseases medicine.symptom |
| Zdroj: | Europe PubMed Central |
| Popis: | Faecal samples were collected from foals between the age of 1 week and 6 months in Belgium, The Netherlands, Germany and Greece. A quantitative direct immunofluorescence assay based on the commercial MERIFLUOR Cryptosporidium/Giardia kit was performed to evaluate the presence of (oo) cysts. Parasite positive samples were genotyped, based on the 18S ribosomal DNA gene and the heat shock protein (HSP70) gene for Cryptosporidium and on the β-giardin gene and the triose phosphate isomerase (TPI) gene for Giardia. In total, 134 foals from Belgium, 44 foals from The Netherlands, 30 foals from Germany and 190 foals from Greece were examined. No Cryptosporidium oocysts were identified in faecal samples from foals in Germany and The Netherlands. In Belgium and Greece, 4.5% and 1.1% of the foals examined were Cryptosporidium positive, respectively, all with a low oocyst excretion ranging from 100 to 2450 oocysts per gram of faeces. For Giardia, 14.2%, 11.4%, 10.0% and 11.6% of the foals in Belgium, The Netherlands, Germany and Greece, respectively, were found to excrete cysts, with a range of 50 up to 4,000,000 cysts per gram of faeces. Younger animals secreted significantly more Giardia cysts than older horses (p |
| Databáze: | OpenAIRE |
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