Screening Natural Content of Water-Soluble B Vitamins in Fish: Enzymatic Extraction, HILIC Separation, and Tandem Mass Spectrometric Determination
| Autor: | K.V. Vishnu, Niladri Sekhar Chatterjee, C.N. Ravishankar, K. Ashok Kumar, Rangasamy Anandan, K.K. Ajeeshkumar, Suseela Mathew, K. R. Remya Kumari |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Riboflavin
Tandem mass spectrometry 01 natural sciences Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Tandem Mass Spectrometry Environmental Chemistry Animals Sample preparation Pharmacology Chromatography Nicotinamide Hydrophilic interaction chromatography 010401 analytical chemistry Extraction (chemistry) Fishes 04 agricultural and veterinary sciences 040401 food science 0104 chemical sciences B vitamins Certified reference materials chemistry Seafood Vitamin B Complex Agronomy and Crop Science Food Science Chromatography Liquid |
| Zdroj: | Journal of AOAC International. 100(3) |
| ISSN: | 1944-7922 |
| Popis: | Despite the potential of LC with tandem MS (MS/MS) in improving sensitivity and selectivity, analytical methods are scarce for the determination of protein-bound and phosphorylated forms of B vitamins in food. This prompted us to develop a method for LC-MS/MS determination of naturally occurring nicotinamide, nicotinic acid, thiamine, pyridoxine, riboflavin, pantothenic acid, biotin, folic acid, and cyanocobalamin in fish. Baseline separation of the vitamins was achieved in a hydrophilic interaction LC condition. An ultrasonication-assisted enzymatic extraction protocol for sample preparation was optimized and validated. The time required for extraction was significantly reduced (to 4 h), while maintaining good extraction efficiency. Acetonitrile content (80%, v/v) in the prepared sample was found to be optimum for excellent peak shape and sensitivity. The dynamic linear range of the vitamins ranged from 2.5 to 500 ng/g, and the regression coefficient values were greater than 0.99. LOQ values ranged from 0.4 to 50 ng/g for the different vitamins. The spike recovery values at 50 and 100 ng/g ranged from 87.5 to 97.5%. The intra- and interday precision values were satisfactory. Accuracy of the developed method was determined by analysis of a Certified Reference Material. The method could also be used for unambiguous determination of the natural content of the target vitamins in fish. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|