Optimization of ultrasound-assisted extraction of phenolic compounds from Sesamum indicum
Autor: | Sourav Chakraborty, Lopamudra Sarma, Raj Kumar Duary, Manas Jyoti Das |
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Rok vydání: | 2019 |
Předmět: | |
Zdroj: | Natural Product Research. 34:1931-1936 |
ISSN: | 1478-6427 1478-6419 |
DOI: | 10.1080/14786419.2018.1564294 |
Popis: | Effective extraction of phyto-biomolecules insures retaining maximum functionality along with higher recovery. In this study, ultrasound-solvent assisted extraction (USAE) was employed for optimal extraction of phyto-biomolecules from Sesamum indicum (sesame) leaves using the approach of Response Surface Methodology (RSM). The optimized condition of 200 W power, 59% methanol concentration with 1:14 g/mL solid–liquid ratio and 15 min of extraction time yielded 367.39 ± 1.85 mg GAE/100 g of total phenolic content, 96.72 ± 3.27% of free radical scavenging activity and 81.20 ± 2.87% of iron chelating activity respectively. The extract consist of essential phytocomponents like gallic acid, chlorogenic acid, and quercetin with lipid peroxidation activities of >50% over incubation time of 48 h. Also, showed antimicrobial activity against various Gram’s negative and positive food borne pathogens. The results of this study implied the importance of USAE for effective and optimum recovery of phyto-biomolecules from Sesame leaves with retained functional properties. |
Databáze: | OpenAIRE |
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