Confocal imaging of autonomic preganglionic neurons in the spinal cord of the caecilian Typhlonectes natans (Amphibia: Gymnophiona)
| Autor: | Daniele Zaccone, Giacomo Zaccone, Karolina A. Budzik, Gioele Capillo, Eugenia Rita Lauriano, Krystyna Żuwała, Michał Kuciel |
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| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Histology sympathetic preganglionic neurons Population confocal microscopy Amphibians Dermis Caecilian amphibians Typhlonectes natans medicine Animals Galanin education Sympathetic preganglionic neurons Neurons education.field_of_study Ganglia Sympathetic Microscopy Confocal biology Cell Biology General Medicine Anatomy Spinal cord biology.organism_classification Immunohistochemistry Ganglion Paravertebral ganglia Confocal microscopy medicine.anatomical_structure Spinal Cord nervous system Caecilian amphibians Confocal microscopy Sympathetic preganglionic neurons Caecilian caecilian amphibians |
| Zdroj: | Acta Histochemica. 116:1399-1406 |
| ISSN: | 0065-1281 |
| DOI: | 10.1016/j.acthis.2014.09.004 |
| Popis: | Little is known about the spinal sympathetic organization in the caecilian amphibians. We examined for the first time the location of sympathetic preganglionic neurons (SPNs) in the spinal cord using a panel of specific markers expressed in SPNs. The SPNs of anuran amphibians form two cell columns segregated mainly in the lateral and medial marginal areas of the central gray matter. In the caecilian Typhlonectes natans immunoreactivity for galanin and ChAT is found in most laterally arranged neurons lying in spinal segments 2–7. They are encircled by TH- and nNOS-immunoreactive nerve fibers. These neurons might project specifically to a population of adrenergic sympathetic postganglionic neurons in paravertebral ganglia and/or non-adrenergic sympathetic postganglionic neurons in the celiac ganglia. However the segmental restriction and target specificity of the neurons of the species studied are not known. As mucous and granular glands in the dermis may represent one of the peripheral targets of the adrenergic ganglion cells and reflect the prominent preganglionic cell columns, an immunohistochemical study was done also on these glands. Retrograde-tracing studies are, however, needed to study the segmental localization of the preganglionic neurons and their projections to the postganglionic neurons in sympathetic ganglia. |
| Databáze: | OpenAIRE |
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