Differentiation and proliferation patterns in human trophoblast revealed by c-erbB-2 oncogene product and EGF-R
| Autor: | Peter Kaufmann, D Zaccheo, Mario Castellucci, H Höfler, J Mühlhauser, Caterina Crescimanno |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Histology Receptor ErbB-2 Pregnancy Trimester Third Proto-Oncogene Mas Pregnancy Proto-Oncogene Proteins Internal medicine Placenta medicine Humans Proliferation Marker Epidermal growth factor receptor In Situ Hybridization reproductive and urinary physiology Syncytium Cytotrophoblast biology Cell Membrane Nuclear Proteins Trophoblast Apical membrane Immunohistochemistry Neoplasm Proteins Trophoblasts Cell biology Basal plasma membrane ErbB Receptors Pregnancy Trimester First Ki-67 Antigen medicine.anatomical_structure Endocrinology embryonic structures biology.protein Female Anatomy Cell Division |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 41:165-173 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/41.2.8093455 |
| Popis: | It is well known that growth factors and proto-oncogenes play a pivotal role in organogenesis as well as in tumor development. The human placenta is a rapidly growing organ which shares some aspects with malignant tumors. We have studied the expression of epidermal growth factor receptor (EGF-R) and the receptor encoded by the c-erbB-2 proto-oncogene in first- and third-trimester human placentas. We compared these expression patterns with that of the proliferation marker Ki-67. By immunohistochemistry, EGF-R was intensively expressed in the villous cytotrophoblast in the first trimester. The apical plasma membrane of the syncytium was weakly stained. In placental villi from the third trimester the reaction product for EGF-R was most intense in single villous cytotrophoblastic cells and along the apical plasma membrane of the syncytium, whereas the basal plasma membrane was much less stained. C-erbB-2 protein product was expressed in the first and third trimesters along the apical membrane of the syncytiotrophoblast. Concerning the extravillous trophoblast in cell islands and cell columns, EGF-R was expressed in the cells proximal to the villous stroma whereas the distal cells were c-erbB-2 positive. The Ki-67 antibody revealed the proliferative character of the villous cytotrophoblast and of the EGF-R-positive extravillous trophoblast. In contrast, most of the c-erbB-2-positive cells were Ki-67 negative. By in situ hybridization, c-erbB-2 transcripts were found in all types of villous and extravillous trophoblast, including those that did not express c-erbB-2 protein product. Our data indicate that EGF-R expression is strongly related to the proliferative trophoblast and, with advancing pregnancy, to the differentiated villous trophoblast.off contrast, expression of c-erB-2 protein product occurs only in more advanced stages of trophoblast differentiation, although transcripts of c-erbB-2 are found in both proliferative and differentiated trophoblast. In addition, the coexpression of EGF-R and c-erbB-2 protein product in the syncytiotrophoblast suggests their involvement in complex regulation of hormones and growth factors. |
| Databáze: | OpenAIRE |
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