Overexpression of Glypican 3 Promotes Proliferation, Regulates Cell Cycle Progression, and Inhibits Apoptosis of Human Fetal Osteoblastic Cell Line 1.19
| Autor: | Xiongzheng Mu, Junyi Yang, Abdulsamad Ghanem, Tianyi Cai, Yingzhi Wu, Ronghu Ke |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Apoptosis Biology Transfection Glypican 3 Cell Line 03 medical and health sciences Glypicans Western blot medicine Humans Viability assay Cell Proliferation Osteoblasts medicine.diagnostic_test Cell growth Cell Cycle General Medicine Cell cycle Molecular biology Cell biology 030104 developmental biology Otorhinolaryngology Cell culture Surgery |
| Zdroj: | Journal of Craniofacial Surgery. 28:1481-1485 |
| ISSN: | 1049-2275 |
| DOI: | 10.1097/scs.0000000000003861 |
| Popis: | Craniosynostosis is a complex disease condition, which involves premature fusion of cranial vault sutures and lacks desirable treatment. Previous studies have demonstrated decreased proliferation rate of osteoblasts and downregulated expression of glypican 3 (GPC3) in syndromic craniosynostosis patients. In this study, quantitative and qualitative analysis were utilized to assess the effect of GPC3 in human fetal osteoblastic cell line, hFOB 1.19. Lentiviral transfection efficiency with green fluorescent protein images was obtained after 72 hours. Western Blot and quantitative real-time polymerase chain reaction analysis results indicated that GPC3 was overexpressed in hFOB 1.19 cells transfected with recombinant lentivirus LV-GPC3-GFP. Cell proliferation was assessed by CCK-8 assay and cell cycle progression and apoptosis were analyzed by flow cytometric assay. Results revealed that GPC3 promoted cell viability, induced cell cycle entry into S phase, and inhibited cell apoptosis. These findings provide novel ideas in understanding the pathogenesis of craniosynostosis. It also provides novel insights in the treatment of craniosynostosis by targeting GPC3. |
| Databáze: | OpenAIRE |
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