Forward chemical genetic screens in Arabidopsis identify genes that influence sensitivity to the phytotoxic compound sulfamethoxazole
| Autor: | Pauline W. Wang, Darrell Desveaux, Karl J. Schreiber, David S. Guttman, Yunchen Gong, Pauline Fung, Ryan S. Austin, Jianfeng Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Sulfamethoxazole Arabidopsis thaliana Mutant Arabidopsis Germination Plant Science Chemical genomics Genes Plant 01 natural sciences Structure-Activity Relationship 03 medical and health sciences lcsh:Botany Sulfanilamides medicine Structure–activity relationship Genetic Testing Genetic Association Studies 030304 developmental biology Genetics 0303 health sciences biology Sulfanilamide biology.organism_classification 3. Good health lcsh:QK1-989 Phenotype Genetic Loci Seedlings Mutation Bacteria Research Article 010606 plant biology & botany medicine.drug Genetic screen |
| Zdroj: | BMC Plant Biology, Vol 12, Iss 1, p 226 (2012) BMC Plant Biology Schreiber, Karl J; Austin, Ryan S; Gong, Yunchen; Zhang, Jianfeng; Fung, Pauline; Wang, Pauline W; et al.(2012). Forward chemical genetic screens in Arabidopsis identify genes that influence sensitivity to the phytotoxic compound sulfamethoxazole. BMC Plant Biology, 12(1), 226. doi: http://dx.doi.org/10.1186/1471-2229-12-226. Retrieved from: http://www.escholarship.org/uc/item/8884b1vf |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/1471-2229-12-226. |
| Popis: | Background The sulfanilamide family comprises a clinically important group of antimicrobial compounds which also display bioactivity in plants. While there is evidence that sulfanilamides inhibit folate biosynthesis in both bacteria and plants, the complete network of plant responses to these compounds remains to be characterized. As such, we initiated two forward genetic screens in Arabidopsis in order to identify mutants that exhibit altered sensitivity to sulfanilamide compounds. These screens were based on the growth phenotype of seedlings germinated in the presence of the compound sulfamethoxazole (Smex). Results We identified a mutant with reduced sensitivity to Smex, and subsequent mapping indicated that a gene encoding 5-oxoprolinase was responsible for this phenotype. A mutation causing enhanced sensitivity to Smex was mapped to a gene lacking any functional annotation. Conclusions The genes identified through our forward genetic screens represent novel mediators of Arabidopsis responses to sulfanilamides and suggest that these responses extend beyond the perturbation of folate biosynthesis. |
| Databáze: | OpenAIRE |
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