Sulphydryl requirement for insulin release from the perfused pancreas
| Autor: | L. Mayer, M. M. C. Landgraf-Leurs, Rüdiger Landgraf |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Dithiothreitol chemistry.chemical_compound Internal medicine Insulin Secretion Internal Medicine medicine Animals Insulin Secretion Pancreas chemistry.chemical_classification Chemistry Rats Perfusion Kinetics Ethacrynic Acid Glucose medicine.anatomical_structure Endocrinology Thiol Beta cell Leucine |
| Zdroj: | Diabetologia. 15:337-342 |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/bf03160999 |
| Popis: | Using the isolated, perfused rat pancreas the importance of sulphydryl groups for the secretory process of insulin was investigated. It was found that ethacrynic acid (EA, 0.075-0.6 mmol/1) caused a dose-dependent, monophasic insulin release. Addition of EA to a glucose-stimulated (20 mmol/1) pancreas led to a sudden increase in hormone release, followed by a dose-dependent inhibition of release, which was not reversible after removal of EA. The same phenomenon was seen in the presence of 20 mmol/1 leucine. Dithiothreitol (DTT, 0.1 and 1 mmol/1) had no effect on basal insulin secretion. Added to a glucose-stimulated pancreas DTT (1 mmol/1) caused a reversible inhibition of insulin release. The persistent inhibitory action of EA on glucose-induced insulin release could be reversed by simultaneous perfusion of EA and DTT. Sequential exposure of a glucose-stimulated pancreas to EA and DTT led to a rapid release of insulin, due to DTT; however, the EA-induced inhibition of insulin secretion could not be prevented. Two kinds of thiol groups in the plasma membrane and in the beta cell might be responsible for the various kinetics of insulin release induced by EA and DTT. |
| Databáze: | OpenAIRE |
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