Development and marker-trait relationships of functional markers for glutamine synthetase GS1 and GS2 homoeogenes in bread wheat

Autor: Magdalena Ruiz, Aida Solé-Medina, Isabel Faci, MARIA ELENA BENAVENTE BARZANA, PATRICIA GIRALDO CARBAJO, Laura Pascual
Přispěvatelé: Ministerio de Ciencia e Innovación (España), Comunidad de Madrid, Universidad Politécnica de Madrid, Pascual, Laura, Solé-Medina, Aida, Faci, Isabel, Giraldo, Patricia, Ruiz, Magdalena, Benavente, Elena
Rok vydání: 2023
Předmět:
Zdroj: Molecular Breeding. 43
ISSN: 1572-9788
1380-3743
DOI: 10.1007/s11032-022-01354-0
Popis: 16 Pág.
GS1 and GS2 genes encode, respectively, the main cytosolic and the plastidic isoforms of glutamine synthetase (GS). In the present study, the wheat GS1 and GS2 homoeogenes located in the A, B and D genome chromosomes have been sequenced in a group of 15 bread wheat varieties including landraces, old commercial varieties and modern cultivars. Phenotypic characterization by multi-environment field trials detected significant effects of specific GS homoeogenes on three of the seven agronomic and grain quality traits analyzed. Based on the gene sequence polymorphisms found, biallelic molecular markers that could facilitate marker-assisted breeding were developed for genes GS1A, GS2A and GS2D. The remaining genes encoding main wheat GS were excluded because of being monomorphic (GS1D) or too polymorphic (GS1B and GS2B) in the sequencing panel varieties. A collection of 187 Spanish bread wheat landraces was genotyped for these gene-based molecular markers. Data analyses conducted with phenotypic records reported for this germplasm collection in López-Fernández et al. (Plants-Basel 10: 620, 2021) have revealed the beneficial influence of some individual alleles on thousand-kernel weight (TKW), kernels per spike (KS) and grain protein content. Furthermore, genetic interactions between GS1A, a cytosolic GS isoform coding gene, and GS2A or GS2D, plastidic GS enzyme coding genes, were found to affect TKW and KS. The finding that some alleles at one locus may mask the effect of positive alleles at hypostatic GS loci should be kept in mind if gene pyramiding strategies are attempted for the improvement of N-use efficiency-related traits.
Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study was funded by the Spanish Ministry of Science and Innovation (Grants No. AGL2016-77149 and PID2019-109089RB-C32 from MCIN/AEI/10.13039/501100011033), by Comunidad de Madrid (Spain; Grant No. S2013/ABI-2717) and by Comunidad de Madrid (Spain) and Structural EU Funds 2014– 2020 (ERDF and ESF) (Grant No. AGRISOST-CM S2018/ AA-4330)
Databáze: OpenAIRE
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