Voltage-sensitive dye recording of glossopharyngeal nerve-related synaptic networks in the embryonic mouse brainstem

Autor: Yoko Momose-Sato, Katsushige Sato
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Neural circuit formation
N.IX
glossopharyngeal nerve
APV
dl-2-amino-5-phosphonovaleric acid
Sensory system
Inferior salivatory nucleus
Biology
Development
CNQX
6-cyano-7- nitroquinoxaline-2
3-dione
CNS
central nervous system
Article
lcsh:RC321-571
03 medical and health sciences
ISN
inferior salivatory nucleus
NTS
nucleus of the tractus solitarius
0302 clinical medicine
VSD
voltage-sensitive dye
medicine
Biological neural network
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
N.X
vagus nerve
Parabrachial Nucleus
General Neuroscience
respiratory system
Optical recording
Voltage-sensitive dye
PBN
parabrachial nucleus
Vagus nerve
030104 developmental biology
medicine.anatomical_structure
nervous system
Glossopharyngeal nerve
Brainstem
Synaptogenesis
Neuroscience
Nucleus
EPSP
excitatory postsynaptic potential
030217 neurology & neurosurgery
Zdroj: IBRO Reports
IBRO Reports, Vol 6, Iss, Pp 176-184 (2019)
ISSN: 2451-8301
Popis: Highlights • We applied voltage-sensitive dye recording to the embryonic mouse brainstem. • We examined functiogenesis of the N.IX-related neural circuits. • Synaptic function in the NTS was first expressed at E12. • Synaptic function in the 2nd/higher-order sensory nuclei emerged at E12–13. • Response areas in the NTS were different between the N.IX and the N.X.
The glossopharyngeal nerve (N.IX) transfers motor and sensory information related to visceral and somatic functions, such as salivary secretion, gustation and the control of blood pressure. N.IX-related neural circuits are indispensable for these essential functions. Compared with the strenuous analysis of morphogenesis, we are only just starting to elucidate the functiogenesis of these neural circuits during ontogenesis. In the present study, we applied voltage-sensitive dye recording to the embryonic mouse brainstem, and examined the functional development of the N.IX-related neural circuits. First, we optically identified the motor nucleus (the inferior salivatory nucleus (ISN)) and the first-order sensory nucleus (the nucleus of the tractus solitarius (NTS)). We also succeeded in recording optical responses in the second/higher-order sensory nuclei via the NTS, including the parabrachial nucleus. Second, we pursued neuronal excitability and the onset of synaptic function in the N.IX-related nuclei. The neurons in the ISN were excitable at least at E11, and functional synaptic transmission in the NTS was first expressed at E12. In the second/higher-order sensory nuclei, synaptic function emerged at around E12-13. Third, by mapping optical responses to N.IX and vagus nerve (N.X) stimulation, we showed that the distribution patterns of neural activity in the NTS were different between the N.IX and the N.X from the early stage of ontogenesis. We discuss N.IX-related neural circuit formation in the brainstem, in comparison with our previous results obtained from chick and rat embryos.
Databáze: OpenAIRE
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