Apical Membrane Antigen 1, a Major Malaria Vaccine Candidate, Mediates the Close Attachment of Invasive Merozoites to Host Red Blood Cells
| Autor: | Gabriele Margos, Graham H. Mitchell, Anton R. Dluzewski, L. H. Bannister, Alan W. Thomas |
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| Rok vydání: | 2004 |
| Předmět: |
Erythrocytes
Immunology Protozoan Proteins Antigens Protozoan Duffy binding proteins Microbiology Epitope Antigen Malaria Vaccines parasitic diseases Animals Plasmodium knowlesi Apical membrane antigen 1 biology Malaria vaccine Antibodies Monoclonal Membrane Proteins biology.organism_classification Macaca mulatta Virology Malaria Rats Cell biology Infectious Diseases Rhoptry neck biology.protein Parasitology Fungal and Parasitic Infections Antibody |
| Zdroj: | Infection and Immunity. 72:154-158 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.72.1.154-158.2004 |
| Popis: | Apical membrane antigen 1 (AMA-1) of Plasmodium merozoites is established as a candidate molecule for inclusion in a human malaria vaccine and is strongly conserved in the genus. We have investigated its function in merozoite invasion by incubating Plasmodium knowlesi merozoites with red cells in the presence of a previously described rat monoclonal antibody (MAb R31C2) raised against an invasion-inhibitory epitope of P. knowlesi AMA-1 and then fixing the material for ultrastructural analysis. We have found that the random, initial, long-range (12 nm) contact between merozoites and red cells occurs normally in the presence of the antibody, showing that AMA-1 plays no part in this stage of attachment. Instead, inhibited merozoites fail to reorientate, so they do not bring their apices to bear on the red cell surface and do not make close junctional apical contact. We conclude that AMA-1 may be directly responsible for reorientation or that the molecule may initiate the junctional contact, which is then presumably dependent on Duffy binding proteins for its completion. |
| Databáze: | OpenAIRE |
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