Biobutanol production from brewer’s spent grain hydrolysates by Clostridium beijerinckii
| Autor: | M. Teresa García-Cubero, Susana Lucas, Mariana Peñuela-Vásquez, Luis Javier Gallego-Morales, Pedro E. Plaza, Mónica Coca |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Environmental Engineering Butanols 020209 energy Bioengineering 02 engineering and technology Raw material 01 natural sciences Hydrolysate chemistry.chemical_compound 1-Butanol 010608 biotechnology Enzymatic hydrolysis 0202 electrical engineering electronic engineering information engineering Monosaccharide Food science Waste Management and Disposal Clostridium beijerinckii chemistry.chemical_classification biology Renewable Energy Sustainability and the Environment Hydrolysis Butanol Sulfuric acid General Medicine biology.organism_classification chemistry Biochemistry Fermentation |
| Zdroj: | Bioresource Technology. 244:166-174 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2017.07.139 |
| Popis: | Brewer's spent grain (BSG) is a promising feedstock for ABE fermentation. Sulfuric acid pretreatment of BSG at pH 1, 121°C and different solid loadings (5-15% w/w) was investigated. Enzymatic hydrolysis and ABE fermentation by Clostridium beijerinckii DSM 6422 of non-washed and washed pretreated BSG were performed to compare monosaccharide release and butanol production. Pretreatment at 15% w/w BSG resulted in higher availability of sugars in both the enzymatic hydrolysates and pretreatment liquid, and overall yields of 75gbutanol/kg BSG and 95gABE/kg BSG were obtained. When the enzymatic hydrolysate from the washed pretreated BSG was fermented, butanol (6.0±0.5g/L) and ABE (7.4±1.0g/L) concentrations were lower compared with 7.5±0.6g/L butanol and 10.0±0.8g/L ABE from a control. The fermentation of the liquid released in the pretreatment at 15% w/w resulted in a butanol production of 6.6±0.8g/L with a total ABE of 8.6±1.3g/L after overliming. |
| Databáze: | OpenAIRE |
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