Long Noncoding RNA Expression Profiling in Normal B-Cell Subsets and Hodgkin Lymphoma Reveals Hodgkin and Reed-Sternberg Cell Specific Long Noncoding RNAs
| Autor: | Anke van den Berg, Jantine Sietzema, Masoumeh Tayari, Lydia Visser, Debora de Jong, Frans G. M. Kroese, Gertrud Kortman, Melanie Winkle, Arjan Diepstra, Martijn Terpstra, Klaas Kok, Pieter Mestdagh, Joost Kluiver |
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| Přispěvatelé: | Translational Immunology Groningen (TRIGR), Stem Cell Aging Leukemia and Lymphoma (SALL), Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics CHROMATIN PATHOGENESIS Germinal center RNA Biology medicine.disease Molecular biology Long non-coding RNA Pathology and Forensic Medicine Lymphoma Transcriptome Gene expression profiling 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure DIFFERENTIATION Reed–Sternberg cell medicine TRANSCRIPTION MEF2C B cell |
| Zdroj: | The American Journal of Pathology, 186(9), 2462-2472. ELSEVIER SCIENCE INC |
| ISSN: | 0002-9440 |
| Popis: | Hodgkin lymphoma (HL) is a malignancy of germinal center (GC) B-cell origin. To explore the role of long noncoding RNAs (lncRNAs) in HL, we studied LncRNA expression patterns in normal B-cell subsets, HL cell lines, and tissues. Naive and memory B cells showed a highly similar lncRNA expression pattern, distinct from GC-B cells. Significant differential expression between I-IL and normal GC-B cells was observed for 475 lncRNA loci. For two validated lncRNAs, an enhanced expression was observed in HL, diffuse large 6-cell lymphoma, and lymphoblastoid cell lines. For a third lncRNA, increased expression levels were observed in HL and part of Burkitt lymphoma cell lines. RNA fluorescence in situ hybridization on primary HL tissues revealed a tumor cell specific expression pattern for all three lncRNAs. A potential cis-regulatory role was observed for 107 differentially expressed lncRNA-mRNA pairs localizing within a 60-kb region. Consistent with a cis-acting role, we showed a preferential nuclear localization for two selected candidates. Thus, we showed dynamic lncRNA expression changes during the transit of normal B cells through the pC reaction and widely deregulated lncRNA expression patterns in HL. Three lncRNAs showed a tumor cell specific expression pattern in HL tissues and might therefore be of value as a biomarker. |
| Databáze: | OpenAIRE |
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