| Autor: |
Xiaoou, Wei, Chao, Liu, Zhihua, Li, Quancai, Sun, Xinai, Zhang, Yanxiao, Li, Wen, Zhang, Jiyong, Shi, Xiaodong, Zhai, Di, Zhang, Xiaobo, Zou |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Chemosphere. 307:135960 |
| ISSN: |
0045-6535 |
| DOI: |
10.1016/j.chemosphere.2022.135960 |
| Popis: |
Thiram has been widely used in agriculture and may invades the food chain, posing a threat to human health. In this research, a label-free electrochemical cell-based biosensor was presented for in vitro toxicity assessment of thiram. HepG2 cells were cultured on poly-l-lysine@gold nano-flowers functionalized indium tin oxide coated glass electrode (PLL@AuNFs/ITO) to serve as biorecognition elements. AuNFs were electrodeposited on ITO to provide an enlarged specific surface area and benefited the output signal amplification. PLL was selected as an effective biocompatible coating material to facilitate cell adhesion and proliferation, thereby realizing one-step recording of electrochemical signals from thiram-treated cells. With the aid of the differential pulse voltammetry method, the fabricated biosensor was applied to assess the cytotoxicity of thiram. Results showed that the cytotoxicity measured by the fabricated biosensor exhibited a linear relationship related to thiram concentration ranging from 5 to 50 μM with a detection limit of 2.23 μM. The IC |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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