Domains of BclA, the major surface glycoprotein of the B. cereus exosporium: glycosylation patterns and role in spore surface properties
| Autor: | Yannick Lequette, Thays Del Lima Dias, Estelle Garénaux, Typhaine Combrouse, Christine Faille, Christian Slomianny, Yann Guérardel, Annette Ronse, Xavier Trivelli |
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| Přispěvatelé: | Laboratoire de Génie des Procédés et Technologie Alimentaires (LGPTA), Institut National de la Recherche Agronomique (INRA), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate, Université de Lille, Sciences et Technologies-Institut National de la Santé et de la Recherche Médicale (INSERM), Génie des Procédés et Technologie Alimentaires (LGPTA), Processus aux Interfaces et Hygiène des Matériaux (PIHM), Université des Sciences et Technologies (Lille 1) (USTL), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Lille, Sciences et Technologies, Université de Lille-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Trivelli, Xavier |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Glycosylation
Rhamnose Surface Properties [SDV]Life Sciences [q-bio] carbohydrates Oligosaccharides Aquatic Science Applied Microbiology and Biotechnology Bacterial Adhesion 03 medical and health sciences chemistry.chemical_compound Bacillus cereus Bacterial Proteins BclA domains [SDV.IDA]Life Sciences [q-bio]/Food engineering Glycosyl [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] 030304 developmental biology Water Science and Technology chemistry.chemical_classification Spores Bacterial 0303 health sciences Membrane Glycoproteins bacillus spore biology 030306 microbiology Chemistry exosporium fungi Exosporium biology.organism_classification Stainless Steel [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Protein Structure Tertiary physicochemistry adhesion Isoelectric point Cereus Biochemistry Mutation CTD Collagen Glycoprotein |
| Zdroj: | Biofouling Biofouling, Taylor & Francis, 2011, 27 (7), pp.751-61 Biofouling, Taylor & Francis, 2011, 27 (7), pp.751-761. ⟨10.1080/08927014.2011.599842⟩ Biofouling, 2011, 27 (7), pp.751-761. ⟨10.1080/08927014.2011.599842⟩ Biofouling, 2011, 27 (7), pp.751-61 |
| ISSN: | 0892-7014 |
| DOI: | 10.1080/08927014.2011.599842⟩ |
| Popis: | International audience; The role of the BclA domains of B. cereus ATCC 14579 was investigated in order to understand the phenomena involved in the interfacial processes occurring between spores and inert surfaces. This was done by (i) creating deletions in the collagen-like region (CLR) and the C-terminal domain (CTD) of BclA, (ii) building BclA proteins with various lengths in the CLR and (iii) modifying the hydrophobic upper surface in the CTD. First, it was demonstrated that the CLR was substituted by three residues already reported in the CLR of B. anthracis, viz. rhamnose, 3-O-methyl-rhamnose, and GalNH(2) residues, while the CTD was also substituted by two additional glycosyl residues, viz. 2-O-methyl-rhamnose and 2,4-O-methyl-rhamnose. Regarding the properties of the spores, both CLR and CTD contributed to the adhesion of the spores, which was estimated by measuring the resistance to detachment of spores adhered to stainless steel plates). CLR and CTD also impacted the hydrophobic character and isoelectric point of the spores. It was then shown that the resistance to detachment of the spores was not affected by the physicochemical properties, but by the CLR length and the presence of hydrophobic amino acids on the CTD. |
| Databáze: | OpenAIRE |
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