Toward Innovative Hemocompatible Surfaces: Crystallographic Plane Impact on Platelet Activation
| Autor: | Zümray Vuslat Parlak, Rafał Zybała, Norina Labude, Karolina Schickle, Anke Aretz, Stephan Rütten, Sabine Neuss, Rainer Telle, Christian Preisinger, Jonas Niessen |
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| Rok vydání: | 2020 |
| Předmět: |
Blood Cells
Materials science Surface Properties Plane (geometry) Biomedical Engineering Endothelial Cells Thrombogenicity Platelet Activation Biomaterials Endothelial stem cell Blood cell Crystallography medicine.anatomical_structure Monolayer medicine Plane orientation Humans Stents Platelet activation Implant |
| Zdroj: | ACS Biomaterials Science & Engineering. 6:6726-6736 |
| ISSN: | 2373-9878 |
| Popis: | The anticoagulation treatment of cardiovascular patients, which is mandatory after implantation of heart valves or stents, has significantly adverse effects on life quality. This treatment can be reduced or even circumvented by developing novel antithrombogenic surfaces of blood-contacting implants. Thus, we aim to discover materials exhibiting outstanding hemocompatibility compared to other available synthetic materials. We present promising surficial characteristics of single crystalline alumina in terms of platelet activation inhibition. In order to elucidate the relation between its crystallographic properties including the plane orientation and blood cell behavior, we examined endothelialization, cytocompatibility, and platelet activation at the blood-alumina interfaces in a controlled experimental setup. We observed that the cell response is highly sensitive to the plane orientation and differs significantly for (0001) and (11-20) planes of Al2O3. Our results reveal for the first time the dependence of platelet activation on crystallographic orientation, which is assumed to be a critical condition controlling the thrombogenicity. Additionally, we used an endothelial cell monolayer as an internal control since endothelial cells have an impact on vessel integrity and implant acceptance. We successfully demonstrate that Al2O3(11-20) exhibits enhanced hemocompatibility in contrast to Al2O3(0001) and is comparable to the physiological endothelial monolayer in vitro. |
| Databáze: | OpenAIRE |
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