Highlighting Cancer Cells with Halochromic Switches
| Autor: | Sicheng Tang, Adrienne S. Brown, Françisco M. Raymo, James N. Wilson, Yang Zhang, Ek Raj Thapaliya |
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| Rok vydání: | 2017 |
| Předmět: |
Fluid Flow and Transfer Processes
Absorption (pharmacology) Aqueous solution Chemistry Process Chemistry and Technology Nanoparticle Bioengineering Protonation 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences Fluorescence 0104 chemical sciences Covalent bond Side chain Organic chemistry 0210 nano-technology Instrumentation Macromolecule |
| Zdroj: | ACS sensors. 2(1) |
| ISSN: | 2379-3694 |
| Popis: | Halochromic coumarin–oxazine prefluorophores and targeting folate ligands can be connected covalently to the side chains of amphiphilic polymers. The resulting macromolecular constructs assemble into nanoparticles in aqueous environments. The prefluorophores do not produce any detectable fluorescence at neutral pH, but are converted into fluorophores with intense visible emission at acidic pH. Protonation opens the oxazine heterocycle to shift bathochromically the coumarin absorption and activate fluorescence with a brightness per nanoparticle approaching 5 × 105 M–1 cm–1. This value translates into a 170-fold enhancement relative to the isolated fluorophores dissolved in organic solvent. The folate ligands direct these multicomponent constructs into acidic intracellular compartments of folate-positive cells, where the prefluorophores switch to the corresponding fluorophores and produce fluorescence. The pH-induced activation of the signaling units ensures negligible background fluorescence from the extra... |
| Databáze: | OpenAIRE |
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