Simple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose
| Autor: | Yoshihiko Uesaka, Shinji Yamasaki, Yoshifumi Takeda, Yoko Otsuka, Junichi Yamaoka, Zaw Lin, Hisao Kurazono |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Gel electrophoresis
Cholera Toxin Escherichia coli Proteins Cholera toxin Bacterial Toxins Galactose Enterotoxin Heat-labile enterotoxin medicine.disease_cause Microbiology Chromatography Affinity chemistry.chemical_compound Enterotoxins Infectious Diseases Column chromatography chemistry Biochemistry Enterotoxigenic Escherichia coli medicine Escherichia coli Sodium dodecyl sulfate |
| Zdroj: | Microbial pathogenesis. 16(1) |
| ISSN: | 0882-4010 |
| Popis: | A simple method for purification of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli and for purification of cholera toxin using immobilized D-galactose column is described. A single run of column chromatography yielded homogeneous toxin as demonstrated by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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