Bio-artificial bone formation model with a radial-flow bioreactor for implant therapy—comparison between two cell culture carriers: porous hydroxyapatite and β-tricalcium phosphate beads

Autor: Tomokazu Matsuura, Mariko Nakamura, Misako Shirai, Kiyoshi Ohkawa, Kiyotsugu Yoshida, Hideki Nomoto, Takahiro Masaki, Yoshihiro Mezaki, Jonghyuk Park, Haruka Maehashi, Mamoru Aizawa
Rok vydání: 2018
Předmět:
Calcium Phosphates
0301 basic medicine
Cancer Research
Artificial bone
medicine.medical_treatment
Cell Culture Techniques
β-Tricalcium phosphate (β-TCP)
Bone grafting
Bone tissue
Hydroxyapatite (HA)
Mice
chemistry.chemical_compound
Bioreactors
0302 clinical medicine
Osteogenesis
Vitamin A
Dental implant
Sulfotransferase (SULT) 1
Cells
Cultured
Vitamin A metabolism
Glucose metabolism
Growth medium
Bone Transplantation
Chemistry
Cell Differentiation
13CO2 exhaust test
Cell biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Sulfotransferases
Porosity
Research Article
Bio-artificial bone
Bioreactor
Models
Biological
03 medical and health sciences
medicine
Animals
Humans
Dental Implants
Osteoblasts
Implant
Cell Biology
Carbon Dioxide
Durapatite
Glucose
030104 developmental biology
Cell culture
Zdroj: Human Cell
ISSN: 1749-0774
Popis: Bone grafting is necessary before dental implant treatment in patients with jaw bone defects. Currently, autologous bone grafting is a major burden on the patient. However, it is impossible to form a sufficient foundation for the implant with a bone-filling agent alone. It is, therefore, necessary to prepare hybrid artificial bone tissue containing osteoblasts and osteoclasts. In this study, mouse MC3T3-E1 pre-osteoblast cells and human embryonic-derived osteoblastic cell line hFOB1.19 were cultured in radial-flow bioreactors (RFB) to form three-dimensional artificial bone filled with porous beads of β-tricalcium phosphate (β-TCP) or hydroxyapatite (HA)—which are clinically used as bone-filling agents—as cell culture carriers. When circulation culturing was performed in the growth medium for the first 10–12 days, glucose consumption was increased in the cultures with HA beads in comparison to the cultures with β-TCP beads. When cultured in the differentiation culture medium during the second half of the culture period, the glucose consumption decreased in the culture with HA beads. A DNA microarray analysis suggested that osteogenesis progressed fast in three-dimensional culture filled with HA beads and that partly differentiation into osteoblasts was prominent in cultures with β-TCP beads. In the growth process of MC3T3-E1 cells, the vitamin A metabolism was also activated, the synthesis and degradation of retinoic acid was enhanced, and the metabolism of the same process decreased at the end of differentiation in three-dimensional cultures. Three-dimensional circulation culture in RFB is considered to be useful for the formation of hybrid bio-artificial bone tissue. Electronic supplementary material The online version of this article (10.1007/s13577-018-0218-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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