Novel Aminoglycoside Resistance Transposons and Transposon-Derived Circular Forms Detected in Carbapenem-Resistant Acinetobacter baumannii Clinical Isolates

Autor: Petra Edquist, Sun Nyunt Wai, Bernt Eric Uhlin, Karin Sjöström, Chinmay Kumar Dwibedi, Anders Johansson, Nabil Karah
Rok vydání: 2016
Předmět:
Acinetobacter baumannii
DNA
Bacterial
0301 basic medicine
Transposable element
Infectious Medicine
medicine.drug_class
Cell- och molekylärbiologi
030106 microbiology
Antibiotics
Cephalosporin
Ceftazidime
Infektionsmedicin
Microbial Sensitivity Tests
Drug resistance
Biology
beta-Lactamases
Microbiology in the medical area
Microbiology
03 medical and health sciences
Bacterial Proteins
Mechanisms of Resistance
Drug Resistance
Multiple
Bacterial
RNA
Ribosomal
16S
Mikrobiologi inom det medicinska området
medicine
Humans
Pharmacology (medical)
Gene
Pharmacology
Molecular Epidemiology
Base Sequence
Molecular epidemiology
Methyltransferases
Sequence Analysis
DNA
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Anti-Bacterial Agents
Aminoglycosides
Infectious Diseases
Carbapenems
DNA Transposable Elements
Cell and Molecular Biology
Acinetobacter Infections
medicine.drug
Zdroj: Antimicrobial Agents and Chemotherapy
ISSN: 1098-6596
0066-4804
DOI: 10.1128/aac.02143-15
Popis: Acinetobacter baumannii has emerged as an important opportunistic pathogen equipped with a growing number of antibiotic resistance genes. Our study investigated the molecular epidemiology and antibiotic resistance features of 28 consecutive carbapenem-resistant clinical isolates of A. baumannii collected throughout Sweden in 2012 and 2013. The isolates mainly belonged to clonal complexes (CCs) with an extensive international distribution, such as CC2 ( n = 16) and CC25 ( n = 7). Resistance to carbapenems was related to bla OXA-23 (20 isolates), bla OXA-24/40-like (6 isolates), bla OXA-467 (1 isolate), and IS Aba1-bla OXA-69 (1 isolate). Ceftazidime resistance was associated with bla PER-7 in the CC25 isolates. Two classical point mutations were responsible for resistance to quinolones in all the isolates. Isolates with high levels of resistance to aminoglycosides carried the 16S rRNA methylase armA gene. The isolates also carried a variety of genes encoding aminoglycoside-modifying enzymes. Several novel structures involved in aminoglycoside resistance were identified, including Tn 6279 , ΔTn 6279 , Ab-ST3- aadB , and different assemblies of Tn 6020 and Tn aphA6 . Importantly, a number of circular forms related to the IS 26 or IS Aba125 composite transposons were detected. The frequent occurrence of these circular forms in the populations of several isolates indicates a potential role of these circular forms in the dissemination of antibiotic resistance genes.
Databáze: OpenAIRE
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