Small RNA Sequencing Reveals Regulatory Roles of MicroRNAs in the Development of Meloidogyne incognita

Autor: Runrun Sun, Robert L. Nichols, Baohong Zhang, Li Qiu, Xiaoping Pan, Huawei Liu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Small RNA
root-knot nematode
cotton infection
01 natural sciences
Catalysis
Deep sequencing
Article
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
deep sequencing
microRNA
Meloidogyne incognita
Root-knot nematode
Animals
small RNA
Tylenchoidea
Physical and Theoretical Chemistry
Molecular Biology
Gene
lcsh:QH301-705.5
Spectroscopy
Regulation of gene expression
Genetics
Life Cycle Stages
biology
Sequence Analysis
RNA
Gene Expression Profiling
Organic Chemistry
Gene Expression Regulation
Developmental
General Medicine
Helminth Proteins
biology.organism_classification
Computer Science Applications
MicroRNAs
030104 developmental biology
Nematode
lcsh:Biology (General)
lcsh:QD1-999
RNA
Small Untranslated
RNA
Helminth
gene regulation
010606 plant biology & botany
Signal Transduction
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 20, Iss 21, p 5466 (2019)
Volume 20
Issue 21
ISSN: 1422-0067
Popis: MicroRNAs (miRNAs) are an extensive class of small regulatory RNAs. Knowing the specific expression and functions of miRNAs during root-knot nematode (RKN) (Meloidogyne incognita) development could provide fundamental information about RKN development as well as a means to design new strategies to control RKN infection, a major problem of many important crops. Employing high throughput deep sequencing, we identified a total of 45 conserved and novel miRNAs from two developmental stages of RKN, eggs and J2 juveniles, during their infection of cotton (Gossypium hirsutum L.). Twenty-one of the miRNAs were differentially expressed between the two stages. Compared with their expression in eggs, two miRNAs were upregulated (miR252 and miRN19), whereas 19 miRNAs were downregulated in J2 juveniles. Nine miRNAs were expressed at high levels, with >
1000 reads per mapped million (RPM) sequenced reads in both eggs and J2 juveniles (miR1, miR124, miR2-3p, miR252, miR279, miR57-5p, miR7904, miR87, and miR92). Three miRNAs were only expressed in eggs (miR4738, miRN3, and miRN5). These differentially expressed miRNAs may control RKN development by regulating specific protein-coding genes in pathways associated with RKN growth and development.
Databáze: OpenAIRE
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