Expression of a human SOCS protein, HSOCP-1, in peripheral blood eosinophils from patients with atopic dermatitis
| Autor: | Kaoru Ogawa, Ryoichi Hashida, Mikito Itoh, Gozoh Tsujimoto, Kenji Matsumoto, Takeshi Nagasu, Yuji Sugita, Masami Miyagawa, Akira Akasawa, Hirohisa Saito, Toshio Katsunuma |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_treatment Immunology Gene Expression Apoptosis Suppressor of Cytokine Signaling Proteins Biology Transfection Cell Line Dermatitis Atopic Suppressor of Cytokine Signaling 1 Protein Complementary DNA Two-Hybrid System Techniques Gene expression medicine Immunology and Allergy Humans Differential display Expressed sequence tag Reverse Transcriptase Polymerase Chain Reaction Intracellular Signaling Peptides and Proteins Nuclear Proteins General Medicine Cell cycle Eosinophil Reverse transcription polymerase chain reaction Eosinophils Repressor Proteins medicine.anatomical_structure Cytokine Trans-Activators Female Carrier Proteins Transcription Factors |
| Zdroj: | International archives of allergy and immunology. 134 |
| ISSN: | 1018-2438 |
| Popis: | To identify new genes related to atopic dermatitis (AD), we screened for differentially expressed genes in peripheral blood eosinophils derived from AD patients and healthy volunteers. RNA was prepared from peripheral blood eosinophils obtained from both AD patients and healthy volunteers, and the expression of various genes was monitored using fluorescent differential display and real-time RT-PCR. One of the expressed sequence tags (ESTs) was expressed at a significantly higher level in AD patients than in healthy volunteers. A full-length cDNA was identified that encoded a human suppressor of cytokine signaling (SOCS) protein, HSOCP-1, also named hASB-8. The expression of HSOCP-1 was increased in cultured peripheral blood eosinophils after IL-4 stimulation, and overexpression of HSOCP-1 caused cell death in an eosinophil cell line, AML14.3D10. p34SEI-1 was identified as a HSOCP-1-interacting protein by a yeast two-hybrid system. It is a protein that also interacts with the cyclin-dependent kinase inhibitor p16INK4, suggesting that HSOCP-1 is involved in cell cycle control and apoptosis. |
| Databáze: | OpenAIRE |
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