Oxidative stress in patients with COPD and pulmonary hypertension
Autor: | Darina Petrasova, Ružena Tkáčová, Branislav Stančák, Zuzana Dorkova, Pavol Joppa |
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Rok vydání: | 2007 |
Předmět: |
Male
medicine.medical_specialty Erythrocytes Hypertension Pulmonary medicine.disease_cause Gastroenterology Pulmonary function testing Pulmonary Disease Chronic Obstructive chemistry.chemical_compound Forced Expiratory Volume Malondialdehyde Internal medicine medicine.artery Tidal Volume medicine Humans Plethysmograph Pulmonary Wedge Pressure Lung Aged chemistry.chemical_classification Glutathione Peroxidase COPD Superoxide Dismutase business.industry Glutathione peroxidase Smoking Stroke Volume General Medicine Carbon Dioxide Middle Aged Catalase medicine.disease Pulmonary hypertension Surgery Oxygen Residual Volume Oxidative Stress chemistry Pulmonary artery Female Lipid Peroxidation business Blood Flow Velocity Oxidative stress |
Zdroj: | Wiener klinische Wochenschrift. 119:428-434 |
ISSN: | 1613-7671 0043-5325 |
Popis: | OBJECTIVE: Oxidative stress plays an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Oxidant/antioxidant imbalance has also been reported in various forms of pulmonary hypertension. The present study aimed to assess systemic oxidative stress, as reflected by serum malondialdehyde (MDA) concentrations and activities of antioxidant enzymes in erythrocytes [glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT)] in patients with and without pulmonary hypertension secondary to COPD. PATIENTS AND METHODS: Seventy-five patients (58 male) with COPD (mean age 65.1 ± 1.2 years; mean smoking history 35.6 ± 3.8 pack-years) were studied. Twenty-one healthy non-smokers served as a control group. Pulmonary function was evaluated with body plethysmography; mean and systolic pulmonary artery pressures (Ppa) were assessed with Doppler echocardiography. Serum concentrations of MDA and activities of GPX, SOD and CAT in washed red blood cells were measured using spectrophotometry. RESULTS: Pulmonary hypertension was present in 28 patients with COPD (systolic Ppa: 46.4 ± 2.3 mmHg; mean Ppa: 26.0 ± 1.9 mmHg) and absent in 47 (systolic Ppa: 22.9 ± 0.8 mmHg; mean Ppa: 13.4 ± 0.6 mmHg). Compared with the healthy control group, all the patients (with or without pulmonary hypertension) had higher serum MDA concentrations (1.5 ± 0.1 versus 2.3 ± 0.1 versus 2.3 ± 0.1 nmol/mL, ANOVA, P < 0.001) and lower erythrocyte GPX activity (51.3 ± 3.2 versus 42.2 ± 2.0 versus 41.3 ± 2.5 U/g Hb, P = 0.029), whereas SOD (1121.1 ± 29.0 versus 1032.6 ± 21.8 versus 1032.7 ± 36.2 U/g Hb, P = 0.063) and CAT activities (4.9 ± 0.2 versus 4.6 ± 0.1 versus 4.7 ± 0.2 U/g Hb; P= 0.454) were similar. No differences were observed in serum MDA concentrations or activities of GPX, SOD and CAT in erythrocytes between COPD patients with and without pulmonary hypertension. CONCLUSION: The study demonstrates the presence of oxidative/antioxidative imbalance in the systemic circulation in patients with COPD: compared with healthy subjects, COPD patients had higher serum MDA concentrations and lower GPX activity in erythrocytes. The magnitudes of the increase in MDA and reduction in GPX activity were similar in COPD patients with pulmonary hypertension and in those with normal pulmonary artery pressures. |
Databáze: | OpenAIRE |
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