Two urinary catecholamine measurement indices for applied stress research: effects of time and temperature until freezing
| Autor: | Norbert K. Semmer, Achim Elfering, Christa Byland, Hans Gerber, Simone Grebner |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Male medicine.medical_specialty Hot Temperature Time Factors Epinephrine Refrigerator car 050109 social psychology Human Factors and Ergonomics Urine Body weight Stress (mechanics) Excretion Behavioral Neuroscience Norepinephrine Animal science Urinary catecholamine 0502 economics and business Congelation Freezing medicine Humans 0501 psychology and cognitive sciences Applied Psychology Chromatography High Pressure Liquid Cryopreservation Chemistry 05 social sciences Reproducibility of Results Surgery Volume (thermodynamics) Research Design Female 050203 business & management Stress Psychological |
| Zdroj: | Human factors. 45(4) |
| ISSN: | 0018-7208 |
| Popis: | Research on stress at work often involves the analysis of urinary adrenaline and noradrenaline. It is usually assumed that samples have to be cooled quickly and stored at refrigerator temperature before freezing. This is often difficult to achieve in field studies. This experimental study therefore tests the robustness of results when samples are not cooled immediately. Samples of 9 men and women, collected at 3 points in time, were immediately frozen, kept for a variable delay in a warm room, or stored in a refrigerator before freezing. Two indices were calculated: (a) the ratio of hormones to liquid volume, period of excretion, and body weight; and (b) the ratio of hormones to urinary creatinine. The reliability of high performance liquid chromatography analysis was satisfactory, as was the comparability of the 2 indices. Unfavorable storage up to 24 hr did not cause bacteria-driven decreases of catecholamines, regardless of storage temperature or sampling time. Results suggest high stability for at least 24 hr without cooling, provided the samples are immediately acidified. Cooling may therefore be handled less restrictively than has been assumed. The application of this research is to facilitate research in settings where samples are collected at different places, such as participants' homes or different workplaces. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|