Regulation of a heterologous glucose transporter promoter in chicken embryo fibroblasts
| Autor: | Martyn K. White, Sarah E. Steane, D Mylott |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
endocrine system
animal structures Monosaccharide Transport Proteins Biophysics Chicken Cells Chick Embryo Transfection Biochemistry Mice Species Specificity Transcriptional regulation Animals RNA Messenger Enhancer Promoter Regions Genetic Molecular Biology Cells Cultured Regulation of gene expression Glucose Transporter Type 1 biology Glucose transporter nutritional and metabolic diseases Cell Biology 3T3 Cells Molecular biology carbohydrates (lipids) Gene Expression Regulation biology.protein GLUT1 hormones hormone substitutes and hormone antagonists Plasmids |
| Zdroj: | Biochemical and biophysical research communications. 252(2) |
| ISSN: | 0006-291X |
| Popis: | GLUT1 is a glucose transporter responsible for increased cellular glucose uptake upon oncogenic transformation or mitogenic stimulation. This is associated with transcriptional activation of the GLUT1 gene. Three regions in the mouse GLUT1 gene mediate this regulation (the promoter and two enhancers). In contrast, chicken GLUT1 transcription is completely uninducible, suggesting either avian signal transduction events are different or the chicken GLUT1 gene lacks these transcriptional control elements. To distinguish between these possibilities, reporter plasmids containing mouse GLUT1 control elements in rodent and avian fibroblasts were compared. These elements function within chicken cells, suggesting that signaling pathways are similar to mouse cells but that the avian GLUT1 gene lacks the control elements necessary to respond to them. |
| Databáze: | OpenAIRE |
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