| Autor: |
John S. Fossey, Aleksandra Jasiulewicz, Conic S, Lee Cooper, Gyorgy Hutvagner, Laszlo Tora, Huy V. Nguyen, Attila Sik, Lucy Wheatley, Sarah Bajan, Ferenc Müller, Divyasree Poovathumkadavil, Joseph W. Wragg, Agnieszka Gambus, Yavor Hadzhiev, H. K. Qureshi |
| Rok vydání: |
2018 |
| Předmět: |
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| Popis: |
Most metazoan embryos commence development with rapid cleavages without zygotic gene expression and their genome activation is delayed until the mid-blastula transition (MBT). However, a set of genes escape global repression during the extremely fast cell cycles, which lack gap phases and their transcription is activated before the MBT. Here we describe the formation and the spatio-temporal dynamics of a distinct transcription compartment, which encompasses the earliest detectable transcription during the first wave of genome activation. Simultaneous 4D imaging of expression of pri-miR430 and zinc finger genes by a novel, native transcription imaging approach reveals a pair of shared transcription compartments regulated by homolog chromosome organisation. These nuclear compartments carry the majority of nascent RNAs and transcriptionally active Polymerase II, are depleted of compact chromatin and represent the main sites for detectable transcription before MBT. We demonstrate that transcription occurs in the S-phase of the cleavage cycles and that the gradual slowing of these cell cycles are permissive to transcription before global genome activation. We propose that the demonstrated transcription compartment is part of the regulatory architecture of nucleus organisation, and provides a transcriptionally competent, supporting environment to facilitate early escape from the general nuclear repression before global genome activation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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