Cellular Hyperplasia in Rats Following Continuous Intravenous Infusion of Recombinant Human Epidermal Growth Factor
| Autor: | F. A. de la Iglesia, Michael R. Bleavins, M. A. Breider, A. W. Gough, J. F. Reindel |
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| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Molecular Sequence Data Respiratory System Urogenital System Cell Count 030226 pharmacology & pharmacy 03 medical and health sciences Harderian gland 0302 clinical medicine Epidermal growth factor Internal medicine Gastrins Mesenchymal cell proliferation medicine Gastric mucosa Animals Humans Amino Acid Sequence Rats Wistar Infusions Intravenous Lamina propria Hyperplasia Epidermal Growth Factor General Veterinary Salivary gland business.industry Body Weight Organ Size 030206 dentistry medicine.disease Recombinant Proteins Small intestine Rats medicine.anatomical_structure Endocrinology Female business Digestive System |
| Zdroj: | Veterinary Pathology. 33:184-194 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/030098589603300208 |
| Popis: | In this study, we determined in vivo morphologic effects of continuous intravenous infusion of recombinant human epidermal growth factor (EGF) in adult Wistar rats. The EGF used consisted of the amino acid residues 1-48 of the human 53-amino-acid EGF molecule, purified from transfected Escherichia coli. Doses of 25, 100, or 250 micrograms/kg body weight were administered using Harvard digital syringe infusion pumps for 4 weeks. At necropsy, the submandibular salivary glands, Harderian glands, liver, kidneys (females only), and ovaries were enlarged and urinary bladders were thickened in 100- and 250-micrograms/kg rats. Numerous tissues of the 100- and 250-micrograms/kg rats contained hyperplastic epithelial cells, and selected organs also had mesenchymal cell proliferation. Epithelial proliferation was most pronounced in the trachea, nasal cavity, nasolacrimal duct, tongue, stomach, small intestine, large intestine, urinary tract, salivary gland ducts, and Harderian gland. Periportal hepatocytes were hypertrophic, correlating with increased liver weight. In addition, mesenchymal cell proliferation was evident in the gastric mucosa lamina propria and in heart valves in 100- and 250-micrograms/kg rats. Increased ovarian weight correlated with increased number and size of corpora lutea and an increased incidence of luteal cysts. Continuous systemic exposure of adult Wistar rats to high doses of EGF resulted in generalized epithelial hyperplasia and tissue-selective mesenchymal proliferation. |
| Databáze: | OpenAIRE |
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