Identification and expression pattern analysis of BoMYB51 involved in indolic glucosinolate biosynthesis from broccoli (Brassica oleracea var. italica)
| Autor: | Jianxin Zhou, Qingyue Yu, Ejiroghene Ruona Evivie, Jing Li, Guodong Hao, Jingying Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Glucosinolates Arabidopsis Biophysics Brassicales Brassica Genes Plant 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Plant defense against herbivory Cloning Molecular Molecular Biology Transcription factor Plant Proteins Reporter gene biology Callose Promoter Cell Biology biology.organism_classification Biosynthetic Pathways 030104 developmental biology chemistry Glucosinolate Transcription Factors 010606 plant biology & botany |
| Zdroj: | Biochemical and Biophysical Research Communications. 501:598-604 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2018.05.058 |
| Popis: | Glucosinolates are a class of amino acid-derived specialized metabolites characteristic of the Brassicales order. Trp derived indolic glucosinolates are essential for the effective plant defense responses to a wide range of pathogens and herbivores. In Arabidopsis, MYB51 is the key transcription factor positively regulates indolic glucosinolate production by activating certain biosynthetic genes. In this study, we report the isolation and identification of a MYB51 from broccoli designated as BoMYB51. Overexpression of BoMYB51 in Arabidopsis increased indolic glucosinolate production by upregulating biosynthetic genes and resulted in enhanced flagellin22 (Flg22) induced callose deposition. The spatial expression pattern and responsive expression of BoMYB51 to several hormones and stress treatments were investigated by expressing the β-glucuronidase (GUS) reporter gene driven by BoMYB51 promotor in Arabidopsis and quantitative real-time PCR analysis in broccoli. Our study provides information on molecular characteristics of BoMYB51 and possible physiological process BoMYB51 may involve. |
| Databáze: | OpenAIRE |
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