miR-21a inhibits decidual cell apoptosis by targeting Pdcd4
| Autor: | Yue Zhang, Rufei Gao, Rong Li, Xueqing Liu, Junlin He, Xuemei Chen, Yubin Ding, Yong-Jiang Zhou, Yi-Xian Wen, Yanqing Geng, Yingxiong Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Medicine (General) Stromal cell mmu-miR-21a QH426-470 Biology Biochemistry 03 medical and health sciences R5-920 0302 clinical medicine Full Length Article microRNA Genetics medicine Decidual cells Molecular Biology Genetics (clinical) Endometrial Stromal Cell Regulation of gene expression 030219 obstetrics & reproductive medicine Decidua Decidualization Cell Biology Cell biology hsa-miR-21 Pdcd4 030104 developmental biology medicine.anatomical_structure Apoptosis Embryo implantation |
| Zdroj: | Genes and Diseases, Vol 8, Iss 2, Pp 171-180 (2021) Genes & Diseases |
| ISSN: | 2352-3042 |
| Popis: | Decidualization of endometrial stromal cells (ESCs) accompanied with embryo implantation is a key process in mammalian reproduction. Evidence suggests that maintenance of decidual cells function is essential. As a critical part in post-transcriptional gene regulation, microRNAs (miRNAs/miR) have been confirmed to be involved in decidualization. However, whether microRNAs regulate decidual cells function has not been reported. Aiming to clarify the role and potential mechanism of miRNAs in decidual cells, artificial induced decidualization model in mice was established. There are 94 differentially expressed miRNAs (≥two-fold change) between decidualized and non-decidualized tissues, including 60 upregulated and 34 downregulated miRNAs. Of the differentially expressed miRNAs, mmu-miR-21a is up-regulated. RT-qPCR also confirmed the up-regulation of mmu-miR-21a following decidualization in vivo and in vitro, and bioinformatic analysis and luciferase activity assay revealed Pdcd4 to be the target gene of mmu-miR-21a. Inhibition of mmu-miR-21a restrained secretory function of decidual cells induced by mESCs, accompanied with increase of Pdcd4 expression and resulted in the increase of cell apoptosis. In addition, we also determined the expression of hsa-miR-21 and Pdcd4 in human proliferative endometrial tissues and decidua tissues. hsa-miR-21 showed higher expression in human decidua tissues compared with proliferative endometrial tissues, while expression of Pdcd4 was contrary to that of hsa-miR-21. Similarly, cell apoptosis increased significantly in human endometrial stromal cell line in response to inhibition of hsa-miR-21. Collectively, we conclude that mmu-miR-21a/hsa-miR-21 may play a key role in regulating the function of decidual cells by inhibiting cell apoptosis through targeting Pdcd4. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|