Lactate efflux and intracellular pH during severe hypoxia in rat cerebral cortex in vitro studied by nuclear magnetic resonance spectroscopy
| Autor: | Risto A. Kauppinen, Tiina-R. M. Pirttilä |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Magnetic Resonance Spectroscopy
Time Factors Intracellular pH In Vitro Techniques Slice preparation medicine Animals Homeostasis Hypoxia Brain Cerebral Cortex Chemistry General Neuroscience Cerebral hypoxia Metabolism Hypoxia (medical) Hydrogen-Ion Concentration medicine.disease Rats Kinetics medicine.anatomical_structure Glucose Biochemistry Cerebral cortex Biophysics Lactates medicine.symptom Cotransporter Energy Metabolism |
| Zdroj: | Neuroscience letters. 178(1) |
| ISSN: | 0304-3940 |
| Popis: | Intracellular pH (pHi) and lactate were monitored in a superfused brain slice preparation using NMR spectrocopy in order to study the role of lactate washout in maintenance of pHi during hypoxia. Data are consistence with a functioning lactate-H+ cotransport in the energetically intact cerebral cortex. This pathway is not, however, linked to regulation of pHi during energy failure with external pH of 6.8 and thus appears not to have physiological impact in H+ homeostasis during cerebral hypoxia. |
| Databáze: | OpenAIRE |
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