Suppression of Alternaria brassicicola infection by volatile compounds from spent mushroom substrates
| Autor: | Mizuki Yokono, Naoki Ube, Atsushi Ishihara, Yasuhito Okuda, Kumiko Osaki-Oka, Shuji Ushijima, Rina Fujita, Emi Fukushima-Sakuno, Kotomi Ueno |
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| Rok vydání: | 2021 |
| Předmět: |
Waste Products
Alternaria brassicicola Volatile Organic Compounds Mushroom biology Hypha Chemistry Arabidopsis Lyophyllum decastes Alternaria Bioengineering Brassica biology.organism_classification Applied Microbiology and Biotechnology food.food Soil Horticulture food Lentinula Pholiota microspora Microspora Leaf spot Agaricales Plant Diseases Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 132:25-32 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2021.03.003 |
| Popis: | Most commercially circulating mushrooms are produced via cultivation using artificially produced mushroom substrates. However, after mushroom harvesting, the disposal of spent mushroom substrates (SMSs) is a serious problem for the mushroom industry owing to the need for a disposal site and the cost involved. Thus, in view of the possibility of recycling SMSs as a soil modifier, we examined the effect of soil mixed with SMSs on the infection of Arabidopsis leaves by Alternaria brassicicola, the causal agent of cabbage leaf spot. The mixing of SMSs used for Hypsizygus marmoreus, Pholiota microspora, Lyophyllum decastes, and Auricularia polytricha into culture soil suppressed the lesion formation caused by A. brassicicola. The defense responses of Arabidopsis were not induced by the culturing of these seedlings in soils containing SMSs. Suppressed lesion formation was observed after the seedlings were treated with volatiles emitted from SMSs that were incubated with soil for 7 days and used for H. marmoreus, P. microspora, L. decastes, A. polytricha, Lentinula edodes, and Cyclocybe cylindracea. The volatiles from the SMSs reduced the elongation of A. brassicicola hyphae. GC-MS analyses of extracts from the SMS containing soils led to the detection of various volatile compounds; among these, skatole, 2,4-di-tert-butylphenol, γ-dodecalactone, butyric acid, guaiacol, 6-amyl-2-pyrone, and 1-octen-3-ol were examined for inhibitory activity on A. brassicicola and found to suppress hyphae elongation. These findings indicate that the antifungal volatile compounds emitted by the SMSs suppress A. brassicicola infection. |
| Databáze: | OpenAIRE |
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