Fecal adrenal hormone patterns during ovulatory and non-ovulatory reproductive cycles in female veiled chameleons (Chamaeleo calyptratus)
Autor: | Robyn H. Pimm, Maya S. Kummrow, Joshua K. R. Tabh, Gabriela F. Mastromonaco, Paula M. Mackie |
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Přispěvatelé: | University of Zurich, Kummrow, Maya S |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Ovulation
medicine.medical_specialty 10253 Department of Small Animals media_common.quotation_subject Metabolite 030209 endocrinology & metabolism Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Corticosterone Internal medicine medicine Animals Testosterone Progesterone 030304 developmental biology media_common 0303 health sciences Estradiol 630 Agriculture Reproduction Lizards biology.organism_classification 1310 Endocrinology Veiled chameleon chemistry 570 Life sciences biology Animal Science and Zoology Female Vitellogenesis 1103 Animal Science and Zoology Glucocorticoid medicine.drug Hormone |
Popis: | The relationship between the reproductive (hypothalamic-pituitary-gonadal; HPG) and adrenal (hypothalamic-pituitary-adrenal; HPA) hormone axes is complex and can vary depending on the species and environmental factors affecting an individual. In an effort to understand this relationship in female veiled chameleons (Chamaeleo calyptratus), the patterns of fecal metabolites of corticosterone (C), estradiol (E), testosterone (T), and progesterone (P) were analyzed by enzyme immunoassay (EIA) during ovulatory (OC; eggs laid) and non-ovulatory cycles (NOC; no eggs laid). Glucocorticoid (GC) metabolites in the fecal extracts were characterized by HPLC and corticosterone EIA performance was assessed by parallelism, accuracy and precision tests. The results indicated that the assay chosen reliably measured the hormone metabolites present in the fecal extracts. Regular, cyclical hormone metabolite patterns consisting of an E peak followed by peaks of T, P and C in close succession were observed during both ovulatory and non-ovulatory cycles; relative levels of P and C, however, were higher during ovulatory cycles. Corticosterone metabolite levels, in particular, increased throughout vitellogenesis and peaked in late vitellogenesis (in non-ovulatory cycles) or around the time of ovulation, and remained elevated throughout the gravid period, falling just prior to oviposition. The results provide evidence of variation in glucocorticoid production throughout different stages of the reproductive cycle, including a role in the ovulatory process; the physiology, however, remains unclear. |
Databáze: | OpenAIRE |
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