Patterns of T-cell reconstitution by assessment of T-cell receptor excision circle and T-cell receptor clonal repertoire after allogeneic hematopoietic stem cell transplantation in leukemia patients ? a study in Chinese patients
| Autor: | De Pei Wu, Qiao Chen Qiu, Wei Rong Chang, Yue Wen Fu, Jian Nong Cen, Ping Zhu, Yu Feng Feng, Zi Ling Zhu |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male China Time Factors Adolescent T-Lymphocytes medicine.medical_treatment T cell Cellular differentiation Receptors Antigen T-Cell chemical and pharmacologic phenomena Hematopoietic stem cell transplantation Biology Immune system medicine Humans Transplantation Homologous Child Leukemia T-cell receptor excision circles T-cell receptor Hematopoietic Stem Cell Transplantation Cell Differentiation Hematology General Medicine Middle Aged medicine.disease Transplantation medicine.anatomical_structure Health Immunology Female |
| Zdroj: | European Journal of Haematology. 79:138-145 |
| ISSN: | 1600-0609 0902-4441 |
| DOI: | 10.1111/j.1600-0609.2007.00885.x |
| Popis: | Objective: Successful allogeneic hematopoietic stem cell transplantation (HSCT) requires reconstitution normal T-cell immunity. Measurement of T-cell receptor excision circles (TRECs) and T-cell receptor β (TCRBV) CDR3 repertoire is a means of quantifying recent thymic T-cell production and reflecting antigen-specific T-cell clones proliferation. Methods: We used real-time quantitative PCR to detect TRECs from 43 Chinese patients who underwent three kind of allo-HSCT without T-cell depletion. RT-PCR was performed to amplify 24 subfamily genes of TCRBV in 24 patients of them. Results: For haploidentical-D group, the TRECs numbers were lower up to 24 months. For matched-sibling donor (MSD) group, the recovery of TRECs was faster than those of other two groups. TRECs values in matched-unrelated donor (MUD) were in the middle. During 2–19 months after transplantation, there were 6–16 BV subfamilies expressed and 33–48% of them were polyclones. The usage rate of TCRBV and percentage of polyclones in haploidentical-D were less than those of other two groups. Twenty-three CDR3 molecules were obtained from nine patients who were potentially associated with GVHD or CMV infection. Conclusions: Analyzing the changes of TCRBV repertoire and measuring TRECs during immune reconstitution would be useful to determine the host’s current immune status and ability of T-cell immune reconstitution and also to find antigen-specific T-cell clones in the three kinds of HSCT. |
| Databáze: | OpenAIRE |
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