THE EFFECT OF PHOTODYNAMIC TREATMENT OF YEAST WITH THE SENSITIZER CHLOROALUMINUM PHTHALOCYANINE ON VARIOUS CELLULAR PARAMETERS
| Autor: | P. J. van den Broek, A. E. Van Gompel, Michel Paardekooper, J. Van Steveninck |
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| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
Indoles Photosensitizing Agents biology Chemistry Substrate (chemistry) General Medicine biology.organism_classification Biochemistry Yeast Kluyveromyces Membrane Enzyme Photochemotherapy Kluyveromyces marxianus Organometallic Compounds Protein biosynthesis Physical and Theoretical Chemistry Incubation |
| Zdroj: | Photochemistry and Photobiology. 62:561-567 |
| ISSN: | 1751-1097 0031-8655 |
| Popis: | Photodynamic treatment of Kluyveromyces marxianus with chloroaluminum-phthalocyanine resulted in loss of clonogenicity. Several parameters were studied to identify targets that could be related to loss of colony-forming capacity. Inhibition of various plasma membrane-bound processes was observed, such as substrate transport and plasma membrane ATPase activity. Moreover, K+ loss from the cells was observed. Photodynamic treatment also reduced the activity of various enzymes involved in energy metabolism, thereby decreasing the cellular ATP level. It will be discussed however that none of these processes is likely to be related directly to loss of clonogenicity. Treatment with phthalocyanine and light resulted in a strong inhibition of the incorporation of 14C-phenylalanine in trichloracetic acid-precipitable material. The induction of the beta-galactoside utilization system was also strongly inhibited. The latter two processes did not recover during incubation, subsequent to photodynamic treatment. It is concluded that photodynamically induced inhibition of protein synthesis is a critical factor contributing to the loss of clonogenicity. |
| Databáze: | OpenAIRE |
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