Photoresponsive polymer–enzyme switches
| Autor: | Edmund A. Larenas, Patrick S. Stayton, Allan S. Hoffman, Tsuyoshi Shimoboji, Timothy Fowler, Samarth Kulkarni |
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| Rok vydání: | 2002 |
| Předmět: |
Models
Molecular chemistry.chemical_classification Acrylamides Multidisciplinary Light biology Polymers Ultraviolet Rays Microfluidics Substrate (chemistry) Active site Nanotechnology Polymer Biological Sciences Conjugated system Enzyme assay Kinetics Enzyme Acrylates Cellulase chemistry biology.protein Organic chemistry Azo Compounds Conjugate |
| Zdroj: | Proceedings of the National Academy of Sciences. 99:16592-16596 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.262427799 |
| Popis: | The ability to photoregulate enzyme activities could provide important new opportunities for development of diagnostic assays, sequential bioprocessing, and lab assays in both traditional and microfluidic formats. We show here that the photoinduced changes in the size and hydration of a “smart” polymer chain coil can be used to regulate substrate access and enzyme activity when conjugated to the enzyme at a specific point just outside the active site. The photoresponsive polymers thus serve jointly as antennae and actuators that reversibly respond to distinct optical signals to switch the polymer–enzyme conjugates on and off, and work when the conjugate is free in solution or when immobilized on magnetic beads. |
| Databáze: | OpenAIRE |
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