Toward protein biomarkers for allergy: CD4+ T cell proteomics in allergic and nonallergic subjects sampled in and out of pollen season
| Autor: | Régine Audran, Blaise Corthésy, Annick Mercenier, Jacqueline Wassenberg, Michael Affolter, Petra Lutter, François Spertini, Stefan Müllner, Stephanie Blum, Andreas Wattenberg, Martin Kussmann, Martin Blüggel |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Proteomics Allergy Proteome T cell Molecular Sequence Data Biology Biochemistry Atopy 03 medical and health sciences Young Adult Antigen medicine Genetic predisposition Hypersensitivity Humans 030304 developmental biology 0303 health sciences Lung 030302 biochemistry & molecular biology Rhinitis Allergic Seasonal General Chemistry Allergens medicine.disease 3. Good health medicine.anatomical_structure Immunology Pollen Female Biomarkers |
| Zdroj: | Journal of proteome research |
| ISSN: | 1535-3907 |
| Popis: | Allergy is an immunological disorder of the upper airways, lung, skin, and the gut with a growing prevalence over the last decades in Western countries. Atopy, the genetic predisposition for allergy, is strongly dependent on familial inheritance and environmental factors. These observations call for predictive markers of progression from atopy to allergy, a prerequisite to any active intervention in neonates and children (prophylactic interventions/primary prevention) or in adults (immunomodulatory interventions/secondary prevention). In an attempt to identify early biomarkers of the "atopic march" using minimally invasive sampling, CD4+ T cells from 20 adult volunteers (10 healthy and 10 with respiratory allergies) were isolated and quantitatively analyzed and their proteomes were compared in and out of pollen season (± antigen exposure). The proteome study based on high-resolution 2D gel electrophoresis revealed three candidate protein markers that distinguish the CD4+ T cell proteomes of normal from allergic individuals when sampled out of pollen season, namely Talin 1, Nipsnap homologue 3A, and Glutamate-cysteine ligase regulatory protein. Three proteins were found differentially expressed between the CD4+ T cell proteomes of normal and allergic subjects when sampled during pollen season: carbonyl reductase, glutathione S-transferase ω 1, and 2,4-dienoyl-CoA reductase. The results were partly validated by Western blotting. |
| Databáze: | OpenAIRE |
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