Administration of a murine diet supplemented with conjugated linoleic acid increases the expression and activity of hepatic uncoupling proteins
| Autor: | Marcos N. Eberlin, Ieda M.R. Prado, Carlos Curti, Amanda Rocha Firmino Pereira, Felippe H.Z. Reis, Fernanda Cardoso, Larissa L. Sá, Sérgio Akira Uyemura, Luciane C. Alberici, Rosana M. Alberici |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Physiology Linoleic acid Conjugated linoleic acid Adipose tissue Mitochondria Liver Mitochondrion Biology medicine.disease_cause chemistry.chemical_compound Mice Internal medicine Endopeptidases medicine Animals Linoleic Acids Conjugated chemistry.chemical_classification Reactive oxygen species Cell Biology Metabolism NAD Endocrinology chemistry Biochemistry Gene Expression Regulation Liver MITOCÔNDRIAS Dietary Supplements lipids (amino acids peptides and proteins) NAD+ kinase Ubiquitin-Specific Proteases Reactive Oxygen Species Oxidative stress |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1573-6881 |
| Popis: | Daily intake of conjugated linoleic acid (CLA) has been shown to reduce body fat accumulation and to increase body metabolism; this latter effect has been often associated with the up-regulation of uncoupling proteins (UCPs). Here we addressed the effects of a CLA-supplemented murine diet (~2 % CLA mixture, cis-9, trans-10 and trans-10, cis-12 isomers; 45 % of each isomer on alternating days) on mitochondrial energetics, UCP2 expression/activity in the liver and other associated morphological and functional parameters, in C57BL/6 mice. Diet supplementation with CLA reduced both lipid accumulation in adipose tissues and triacylglycerol plasma levels, but did not augment hepatic lipid storage. Livers of mice fed a diet supplemented with CLA showed high UCP2 mRNA levels and the isolated hepatic mitochondria showed indications of UCP activity: in the presence of guanosine diphosphate, the higher stimulation of respiration promoted by linoleic acid in mitochondria from the CLA mice was almost completely reduced to the level of the stimulation from the control mice. Despite the increased generation of reactive oxygen species through oxi-reduction reactions involving NAD(+)/NADH in the Krebs cycle, no oxidative stress was observed in the liver. In addition, in the absence of free fatty acids, basal respiration rates and the phosphorylating efficiency of mitochondria were preserved. These results indicate a beneficial and secure dose of CLA for diet supplementation in mice, which induces UCP2 overexpression and UCP activity in mitochondria while preserving the lipid composition and redox state of the liver. |
| Databáze: | OpenAIRE |
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