MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression
| Autor: | Hilda Jiménez-Wences, Miriam Ramírez-Carrillo, Gabriela Elizabeth Campos-Viguri, Dinorah Nashely Martínez-Carrillo, Oscar Peralta-Zaragoza, Marco Antonio Jiménez-López, Eduardo Castañeda-Saucedo, Alma Edith Longinos-González, César López‑Camarillo, Carlos Alberto Castañón-Sánchez, Gloria Fernández-Tilapa |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
C-Met Science lcsh:Medicine Uterine Cervical Neoplasms GAB1 Biology Response Elements Article law.invention Malignant transformation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement law Cell Line Tumor microRNA medicine Humans Neoplasm Invasiveness Neoplasm Metastasis lcsh:Science 3' Untranslated Regions Gene Cell Proliferation Multidisciplinary Oncogene lcsh:R Cancer Proto-Oncogene Proteins c-met medicine.disease Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology chemistry 030220 oncology & carcinogenesis Cervical cancer Cancer research Medicine Suppressor lcsh:Q Female Algorithms |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-60143-x |
| Popis: | Malignant transformation and progression in cancer is associated with the altered expression of multiple miRNAs, which are considered as post-transcriptional regulators of genes participating in various cellular processes. Although, it has been proposed that miR-23b-3p acts as a tumor suppressor in cervical cancer (CC), not all the pathways through which it alters the cellular processes have been described. The present study examines whether miR-23b-3p directly represses the c-Met expression and that consequently modifies the proliferation, migration and invasion of C33A and CaSki cells. c-Met has five microRNA response elements (MREs) for miR-23b-3p in the 3′-UTR region. The ectopic overexpression of miR-23b-3p significantly reduces c-Met expression in C33A and CaSki cells. The overexpression of miR-23b-3p reduces proliferation, migration and invasion of CaSki cells and the proliferation and invasion in C33A cells. In CaSki cells, the activation of Gab1 and Fak, downstream of c-Met, is reduced in response to the overexpression of miR-23b-3p. Together, the results in the present study indicate that miR-23b-3p is a tumor suppressor that modulates the progression of CC via post-transcriptional regulation of the c-Met oncogene. |
| Databáze: | OpenAIRE |
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