Norepinephrine transporter expressed on mammary epithelial cells incorporates norepinephrine in milk into the cells
| Autor: | Tomoji Maeda, Takeshi Chiba, Akira Takaguri |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Restraint
Physical 0301 basic medicine medicine.medical_specialty Mammary gland Biophysics CD1 Biological Transport Active Breast milk Biochemistry Cell Line Mice Norepinephrine 03 medical and health sciences Mammary Glands Animal 0302 clinical medicine Pregnancy Stress Physiological Internal medicine Lactation medicine Animals Humans RNA Messenger Mammary Glands Human Receptor Molecular Biology Cells Cultured Norepinephrine Plasma Membrane Transport Proteins Milk Human biology Chemistry Caseins Epithelial Cells Cell Biology Apical membrane Milk 030104 developmental biology medicine.anatomical_structure Endocrinology Mammary Epithelium Norepinephrine transporter 030220 oncology & carcinogenesis biology.protein Female Receptors Adrenergic beta-2 |
| Zdroj: | Biochemical and Biophysical Research Communications. 545:1-7 |
| ISSN: | 0006-291X |
| Popis: | Mammary epithelial cells synthesize and secrete norepinephrine (NE) into breast milk to regulate β-casein expression through the adrenergic β2 receptor. We investigated the expression, localization, and roles of NE transporter (NET) in the mammary epithelium during lactation. mRNA and protein levels of NET were determined in primary normal human mammary epithelial cells (pHMECs) and non-malignant human mammary epithelial MCF-12A cells. In nursing CD1 mice, NET localized to the apical membranes of the mammary epithelium. The intracellular NE content of pHMECs incubated with NE increased. Although the β-casein concentration in milk was slightly higher at day 10 than at day 2 of lactation, the NE concentration and lactation-related proteins were only slightly changed on days 2-10. Restraint stress increased the NE concentration in milk from nursing mice and NET protein levels were significantly higher than in non-stressed nursing mice. NET is expressed on the apical membrane of mammary epithelial cells and incorporates NE in milk into cells, potentially regulating the NE concentration in milk. |
| Databáze: | OpenAIRE |
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