Genetic recombination in Escherichia coli B III. The influence of experimental conditions on the transfer of unselected markers

Autor:	P. G. de Haan
Rok vydání:	1955
Předmět:	Recombination Genetic Genetics Methionine Locus (genetics) Plant Science General Medicine Biology medicine.disease_cause Genetic recombination chemistry.chemical_compound chemistry Insect Science Escherichia coli medicine Animal Science and Zoology Recombination
Zdroj:	Genetica. 27:364-376
ISSN:	1573-6857 0016-6707
DOI:	10.1007/bf01664168
Popis:	a. The transfer-percentage of some unselected markers can be influenced by experimental conditions such as the nature of the culture medium on which the F− parent is grown. This effect varies for different loci. b. Introduction of other markers may abolish the effect mentioned undera. Cultural conditions can therefore influence the apparent degree of linkage. c. Addition of methionine to recombination plates can materially alter the percentage of transfer of unselected markers. This effect should be taken into consideration in studying linkage to the methionine locus. For such studies the usual procedure of comparing prototrophs (m+) from recombinations on minimal medium with recombinants (m−) on methionine supplemented plates is dangerous. To arrive at more reliable linkage data the m+ class should also be derived from supplemented plates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e604709e202a3b2f81ce1d7cd6b7052 https://doi.org/10.1007/bf01664168 Zobrazit plný text záznamu Full text from SpringerLink