Autophagy facilitates secretion and protects against degeneration of the Harderian gland
| Autor: | Marion Gröger, Barbara Lengauer, Manfred Fobker, Heidemarie Rossiter, Christine Hartmann, Caterina Barresi, Johannes Pammer, Ulrich Koenig, Guenter P. Resch, Marlene Brandstetter, Gabriele Plenz |
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| Rok vydání: | 2014 |
| Předmět: |
RFU
relative fluorecent units MG132 Harderian gland SQSTM1/p62 Atg7 autophagy related 7 DMSO dimethyl sulfoxide Mice MAP1LC3A/B (LC3) microtubule-associated protein 1 light chain 3 α/β PCR polymerase chain reaction Lipid droplet BCA bicinchoninic acid assay BODIPY boron-dipyrromethene fluorescent dye GFP green fluorescent protein PLIN2 perilipin 2 education.field_of_study Atg12 autophagy related 12 SQSTM1 sequestosome 1/p62 ELISA enzyme-linked immunosorbent assay lipotoxicity KLICK keratosis lineariz with ichthyosis congenita and sclerosing keratoderma Aggresome medicine.anatomical_structure LD Lipid drops lysosome Proteasome Inhibitors perilipin 2/adipophilin multilamellar bodies PARP poly (ADP-ribose) polymerase keratinocytes FC free cholesterol autophagy TBS-T Tris buffered saline with Tween 20 MG312 synthetic peptide Z-Leu-Leu-Leu-al Perilipin 2 Basic Science Research Papers degenerative diseases Biology Sequestosome 1 aggresome Lysosome medicine Animals f floxed Secretion education Molecular Biology Cell Nucleus LSM laser scanning microscope TLC thin layer chromatography KRT14 Keratin 14 proteasome inhibitor Endoplasmic reticulum cholesterol ER edoplasmic reticulum Epithelial Cells Cell Biology Molecular biology UV ultraviolet Vacuoles aggregates biology.protein HaGl Harderian gland ORO oil red O palmitate BSA bovine serum albumin Lysosomes Cre Cre recombinase |
| Zdroj: | Autophagy |
| ISSN: | 1554-8635 1554-8627 |
| DOI: | 10.4161/15548627.2014.978221 |
| Popis: | The epithelial derived Harderian gland consists of 2 types of secretory cells. The more numerous type A cells are responsible for the secretion of lipid droplets, while type B cells produce dark granules of multilamellar bodies. The process of autophagy is constitutively active in the Harderian gland, as confirmed by our analysis of LC3 processing in GFP-LC3 transgenic mice. This process is compromised by epithelial deletion of Atg7. Morphologically, the Atg7 mutant glands are hypotrophic and degenerated, with highly vacuolated cells and pyknotic nuclei. The mutant glands accumulate lipid droplets coated with PLIN2 (perilipin 2) and contain deposits of cholesterol, ubiquitinated proteins, SQSTM1/p62 (sequestosome 1) positive aggregates and other metabolic products such as porphyrin. Immunofluorescence stainings show that distinct cells strongly aggregate both proteins and lipids. Electron microscopy of the Harderian glands reveals that its organized structure is compromised, and the presence of large intracellular lipid droplets and heterologous aggregates. We attribute the occurrence of large vacuoles to a malfunction in the formation of multilamellar bodies found in the less abundant type B Harderian gland cells. This defect causes the formation of large tertiary lysosomes of heterologous content and is accompanied by the generation of tight lamellar stacks of endoplasmic reticulum in a pseudo-crystalline form. To test the hypothesis that lipid and protein accumulation is the cause for the degeneration in autophagy-deficient Harderian glands, epithelial cells were treated with a combination of the proteasome inhibitor and free fatty acids, to induce aggregation of misfolded proteins and lipid accumulation, respectively. The results show that lipid accumulation indeed enhanced the toxicity of misfolded proteins and that this was even more pronounced in autophagy-deficient cells. Thus, we conclude autophagy controls protein and lipid catabolism and anabolism to facilitate bulk production of secretory vesicles of the Harderian gland. |
| Databáze: | OpenAIRE |
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