Species and sex differences of aflatoxin B1-induced glutathione S-transferase placental form in single hepatocytes
| Autor: | Kojiro Tsuji, Kiyomi Sato, Prabhakar D. Lotlikar, Masatomo Kimura, Kiyoko Lehmann, Atsushi Horiuchi, Prathima Gopalan |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Aflatoxin B1 Placenta medicine.medical_treatment Intraperitoneal injection Hamster chemistry.chemical_compound Pregnancy Cricetinae Internal medicine medicine Animals Buthionine sulfoximine Cells Cultured Carcinogen Glutathione Transferase Sex Characteristics Mesocricetus biology Glutathione Rats Inbred F344 Rats Isoenzymes medicine.anatomical_structure Endocrinology Glutathione S-transferase Liver Oncology chemistry Enzyme Induction Hepatocyte Toxicity biology.protein Female |
| Zdroj: | Cancer Letters. 66:249-254 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/0304-3835(92)90254-s |
| Popis: | Species and sex differences of aflatoxin B1 (AFB1)-induced glutathione S-transferase placental form (GST-P) positive single hepatocytes have been investigated 48 h after an intraperitoneal injection of AFB1 to young male and female Fischer rats (2 mg AFB1/kg body wt) and male Syrian golden hamsters (6 mg AFB1/kg body wt). The presence of GST-P positive hepatocytes was examined by the immunohistochemical method. Male rats formed three times as many AFB1-induced GST-P positive hepatocytes as females. Pretreatment of both male and female rats with an inhibitor of GSH synthesis, buthionine sulfoximine (BSO) (4 mmol/kg body wt), 2 h and 4 h before AFB1 injection increased AFB1-induced GST-P positive hepatocytes by about 120% above the controls. Male hamsters formed several-fold less AFB1-induced GST-P positive hepatocytes than male rats. Pretreatment with BSO did not increase AFB1-induced GST-P positive hepatocytes in hamsters even though it produced an increase in hepatic necrosis. It appears that GSH and GSH S-transferases play an important role in modulating hepatic AFB1-DNA binding and AFB1-induced GST-P positive hepatocytes in rats and hamsters. |
| Databáze: | OpenAIRE |
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