Procalcitonin measurement using antibody-conjugated fluorescent microspheres distinguishes atypical bacterial meningitis from viral encephalitis in children
| Autor: | Si Lin, Yan Wang, Yu Ai, Kang Du, Xingmiao Liu, Xinquan Liu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Biophysics Fluorescent Antibody Technique 01 natural sciences Biochemistry Gastroenterology Procalcitonin Meningitis Bacterial Antigen-Antibody Reactions Diagnosis Differential 03 medical and health sciences Cerebrospinal fluid Fluorescent microspheres Internal medicine medicine Humans Encephalitis Viral Child Molecular Biology 030304 developmental biology 0303 health sciences biology Receiver operating characteristic business.industry Viral encephalitis 010401 analytical chemistry Cell Biology medicine.disease Microspheres 0104 chemical sciences ROC Curve Case-Control Studies biology.protein Bacterial meningitis Antibody business Procalcitonin Measurement hormones hormone substitutes and hormone antagonists Biomarkers |
| Zdroj: | Analytical biochemistry. 626 |
| ISSN: | 1096-0309 |
| Popis: | Examination of cerebrospinal fluid in atypical bacterial meningitis (ABM) is similar to that of viral encephalitis (VE), so ABM can easily be misdiagnosed as VE, which can delay diagnosis and treatment. We developed a simple, rapid hand-held lateral flow immunoassay detection system based on fluorescent microspheres (FMS) for procalcitonin (PCT) detection, which provides an indicator to differentiate between ABM and VE. With this novel method, the antigen-antibody reaction systems involve different species, making the test strips more stable than those utilizing one species. The strips exhibited a wide dynamic range (0.04–50 ng/mL) and good sensitivity (0.03 ng/mL). The function of PCT in the identification of ABM and VE in children was further studied. A significant difference in PCT levels was observed between the ABM and VE groups (P = 0.00) and between the ABM and the normal control groups (P = 0.00). PCT levels were not significantly different between the VE and normal control groups (P = 0.30). The area under the receiver operating characteristic curve of PCT for the diagnosis of ABM was 0.95. These findings collectively indicate the usefulness of the PCT detection method based on FMS for clinically differentiating between ABM and VE. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect