Respiratory Syncytial Virus G Protein CX3C Motif Impairs Human Airway Epithelial and Immune Cell Responses
| Autor: | Fahad M. Malik, Seyhan Boyoglu-Barnum, Tatiana Chirkova, Steven P. Trau, Antonius G. P. Oomens, Larry J. Anderson, Kelsey A. Gaston |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Chemokine
Immunology Amino Acid Motifs CX3C Chemokine Receptor 1 Respiratory Syncytial Virus Infections Biology Adaptive Immunity Microbiology Virus Viral Proteins Immune system Immunity Interferon Virology medicine Humans Epithelial Cells Acquired immune system Chemokines CX3C Immunity Innate medicine.anatomical_structure Insect Science Respiratory Syncytial Virus Human biology.protein Leukocytes Mononuclear Pathogenesis and Immunity Tumor necrosis factor alpha Receptors Chemokine Interferons Memory T cell medicine.drug |
| Popis: | Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory infection in infants and young children and causes disease in the elderly and persons with compromised cardiac, pulmonary, or immune systems. Despite the high morbidity rates of RSV infection, no highly effective treatment or vaccine is yet available. The RSV G protein is an important contributor to the disease process. A conserved CX3C chemokine-like motif in G likely contributes to the pathogenesis of disease. Through this motif, G protein binds to CX3CR1 present on various immune cells and affects immune responses to RSV, as has been shown in the mouse model of RSV infection. However, very little is known of the role of RSV CX3C-CX3CR1 interactions in human disease. In this study, we use an in vitro model of human RSV infection comprised of human peripheral blood mononuclear cells (PBMCs) separated by a permeable membrane from human airway epithelial cells (A549) infected with RSV with either an intact CX3C motif (CX3C) or a mutated motif (CX4C). We show that the CX4C virus induces higher levels of type I/III interferon (IFN) in A549 cells, increased IFN-α and tumor necrosis factor alpha (TNF-α) production by human plasmacytoid dendritic cells (pDCs) and monocytes, and increased IFN-γ production in effector/memory T cell subpopulations. Treatment of CX3C virus-infected cells with the F(ab′) 2 form of an anti-G monoclonal antibody (MAb) that blocks binding to CX3CR1 gave results similar to those with the CX4C virus. Our data suggest that the RSV G protein CX3C motif impairs innate and adaptive human immune responses and may be important to vaccine and antiviral drug development. |
| Databáze: | OpenAIRE |
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